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A4955

Sigma-Aldrich

Alkaline Phosphatase Stabilizing Buffer

liquid

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

sterility

non-sterile

form

liquid

technique(s)

ELISA: suitable

storage temp.

2-8°C

General description

Alkaline Phosphatase Stabilizing Buffer can extend the shelf life of alkaline phosphate conjugates.

Application

Alkaline Phosphatase (ALP) Stabilizing Buffer may be used in ALP assay in osteoblast cell protein lysates. It may also be used as a component of antibody-enzyme conjugate dialysis buffer.
Alkaline Phosphatase Stabilizing Buffer has been tested in ELISA systems. The product has been specially made for the stable dilution of alkaline phosphatase-antibody conjugates.It can be used for a series of experiments to achieve consistent results.

Components

Stabilizing compounds in Tris buffered saline, pH 8.0, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

The degree of stability realized is highly dependent upon the conjugate tested. Trials using each particular conjugate should be performed to determine the degree of stability that can be expected. It is recommended that this buffer constitute ≥95% of the final solution.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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