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DL-Dithiothreitol solution

1 M in H2O

Synonym(s):

(±)-threo-1,4-Dimercapto-2,3-butanediol solution, DTT

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About This Item

Empirical Formula (Hill Notation):
C4H10O2S2
CAS Number:
Molecular Weight:
154.25
Beilstein:
1719757
MDL number:
UNSPSC Code:
12161504
PubChem Substance ID:
NACRES:
NA.25

form

liquid

Quality Level

reaction suitability

reagent type: reductant

concentration

1 M in H2O

density

1.04 g/mL at 20 °C
1.035 g/mL at 25 °C

SMILES string

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

InChI key

VHJLVAABSRFDPM-QWWZWVQMSA-N

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General description

Dithiothreitol (DTT) is used in proteomics applications to maintain sulfhydryl (-SH) groups in the reduced state and for quantitative reduction of disulfide (-S-S-) groups. It is a frequently used reagent in buffers due to its ability to reduce the oxidation state of a protein sample and preserves enzymatic activity. DTT is oxidized to the cyclic disulfide during the reduction of other disulfides in the solution.
Dithiothreitol is an effective reducing agent for protein analysis. The sealed-ampule format is convenient and easy to use when preparing samples for electrophoresis or other applications and avoids waste and loss of potency.

Application

DL-Dithiothreitol solution has been used:
  • in western blot analysis
  • as a supplement in lysis buffer to study its effects on sperm lysis
  • as a component of cold homogenization buffer, and chilled wash buffer for the isolation of nuclei from frozen rat tissues
  • in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), chromatography, modification of cysteine-containing compounds

Features and Benefits

Low toxicity and reduced odor compared to other thiol compounds.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Boseon Kim et al.
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More than half of the proteome from mandibular glands in caterpillars is represented by chemosensory proteins. Based on sequence similarity, these proteins are putative transporters of ligands to gustatory receptors in sensory organs of insects. We sought to determine whether

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