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217669

Sigma-Aldrich

Anti-CD28 Mouse mAb (ANC28.1/5D10)

liquid, >95% (immunoglobulin, SDS-PAGE), clone ANC28.1/5D10

Synonym(s):

Anti-CD28 Antibody, CD28 Detection Antibody, Mouse Anti-CD28

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UNIT
$2,030.00

$2,030.00

Estimated to ship onMay 18, 2025

Validation Kit with 3 columns out of 3 different sorbent lots
To order products, please contact your local dealer.
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UNIT
$2,030.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

$2,030.00

Estimated to ship onMay 18, 2025

Validation Kit with 3 columns out of 3 different sorbent lots
To order products, please contact your local dealer.

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

ANC28.1/5D10, monoclonal

Assay

>95% (immunoglobulin, SDS-PAGE)

form

liquid

contains

500 μg/mL gentamycin sulfate as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

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8.15106

2-Amino-4,6-dichlorobenzoic acid

2,5-Dichlorobenzoic acid for synthesis

8.18834

2,5-Dichlorobenzoic acid

2-Chlorobenzoyl chloride for synthesis

8.21989

2-Chlorobenzoyl chloride

4,5-Difluoroanthranilic acid 97%

399760

4,5-Difluoroanthranilic acid

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

storage temp.

2-30°C

storage temp.

2-30°C

storage temp.

2-30°C

storage temp.

-

form

powder

form

powder

form

liquid

form

-

pH

2.3 (20 °C in H2O)

pH

-

pH

-

pH

-

General description

Anti-CD28, mouse monoclonal, clone ANC28.1/5D10, recognizes CD28 on human T cells & human CD28 transfected BHK cells. It is validated for FC, IP, IHC on frozen sections, and T cell co-stimulation.
Protein A purified mouse monoclonal antibody. Recognizes the CD28 protein.
Recognizes CD28 on human T cells and BHK cells transfected with human CD28.

Immunogen

Human
human CD28 Ig fusion protein and HPB-MLT human tumor cells

Application

Flow Cytometry (5 µg/ml)

Frozen Sections (see comments)

Immunoprecipitation (see comments)

T cell Co-stimulation (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.

Reconstitution

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Other Notes

Kariv, I., et al. 1996. J. Immunol.157, 29.
61st Forum in Immunology. 1995. Res. Immunol.146, 127.
Jenkins, M.K., and Johnson, J.G. 1993. Curr. Opin. Immunol.5, 361.
Monomeric and cross-linked antibody will stimulate expression of IL-2 from CD28+ cells. FACS was performed on 5x105 cultured HPB-MLT cells using anti-CD28 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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