05-1116

Anti-VEGF Antibody, clone CH-10

clone CH-10, from mouse, purified by affinity chromatography

• Synonym(s): Vascular permeability factor, vascular endothelial growth factor, vascular endothelial growth factor A, vascular endothelial growth factor isoform VEGF165
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: CH-10, monoclonal
• purified by: affinity chromatography
• species reactivity: human, rat
• technique(s): western blot: suitable
• isotype: IgG1
• NCBI accession no.: NP_033531.3
• UniProt accession no.: P15692
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... VEGFA(7422)

Description

General description

VEGF (Vascular endothelial growth factor, VEGFA, VEGF-A), a dimeric ligand, is among the most potent angiogenic mitogens. VEGF is secreted by tumor cells and other cells exposed to hypoxia. VEGF is a highly specific mitogen for vascular endothelial cells. Seven VEGF isoforms (a, b, c, d, e, f, g) are generated as a result of alternative splicing from a single VEGF gene. All seven isoforms differ in their molecular mass and in biological properties such as their ability to bind to cell-surface heparan-sulfate proteoglycans. The expression of VEGF is potentiated and is secreted by tumor cells in response to hypoxia, by activated oncogenes, and by a variety of cytokines.

Specificity

This antibody recognizes human and rat VEGF, other variants not tested.

Immunogen

Recombinant mouse VEGF.

Application

Anti-VEGF Antibody, clone CH-10 is an antibody against VEGF for use in WB.

Research Category
Signaling

Research Sub Category
Growth Factors & Receptors

Quality

Evaluated by Western Blot in Jurkat cell lysate.
Western Blot Analysis: 1:1000 dilution of this antibody detected VEGF on 10 µg of Jurkat cell lysate.

Target description

12/16/19 kDa

Linkage

Replaces: MAB1665

Physical form

Affinity purified

Liquid in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6 containing 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Jurkat cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable