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Supelco

HybridSPE® DPX Tip

Universal format, Tip volume 1 mL, box of 96 tips, bed weight 30 mg

Synonym(s):

HybridSPE® DPX SPE Tips

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About This Item

UNSPSC Code:
41104008
NACRES:
NB.21
Pricing and availability is not currently available.

Product Name

HybridSPE® DPX Tip, weight 30 mg (bed), Universal format, tip volume 1 mL

product line

HybridSPE®

composition

HybridSPE® sorbent

packaging

box of 96 tips

technique(s)

solid phase extraction (SPE): suitable

tip volume

1 mL

weight

30 mg (bed)

matrix active group

Zirconia-based phase

application(s)

food and beverages

compatibility

Universal format

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General description

DPX stands for Dispersive Pipette EXtraction. HybridSPE DPX Tips are pipette tips that incorporate loosely contained HybridSPE sorbent material that is mixed with the sample solution to accomplish solid phase extraction. HybridSPE technology is a simple and generic sample prep platform designed for the gross level removal of endogenous phospholipid interferences from biological plasma and serum prior to LC/MS or LC/MS/MS analysis.
In this simple technique, biological plasma or serum is first subjected to protein precipitation via the addition and mixing of acidified acetonitrile. Precipitated proteins are then removed by centrifugation and the resulting supernatant is extracted using the HybridSPE DPX tip which acts as a chemical filter that specifically targets the removal of endogenous sample phospholipids.
The phospholipid retention mechanism is based on a highly selective Lewis acid-base interaction between the proprietary zirconia ions functionally bonded to the HybridSPE® stationary phase and the phosphate moiety consistent with all phospholipids. The resulting eluent is ready for immediate LC/MS or LC/MS/MS analysis.

We offer an array of tips that are compatible with the most common liquid handling platforms and pipettors on the market: Tecan®, Hamilton®, INTEGRA, and a Universal format as well.
What size tips do I need?
There are two versions of the DPX tips available for the HybridSPE-PL. The standard HybridSPE DPX 50 mg tips are designed for use with biological sample volumes between 100-300 µL, while the HybridSPE DPX 30 mg tips are designed for use with biological sample volumes between 30-100 µL.

Guideline for 30 mg tips - plasma/serum sample: 30-100 µL; precipitating agent: 90-300 µL


Guideline for 50 mg tips - plasma/serum sample: 100-300 µL; precipitating agent: 300-900 µL

Features and Benefits

  • Minimal elution solvent volumes
  • Rapid extraction times (less than 3 minutes/sample)
  • High extraction efficiencies
  • Easy to perform extractions
  • Lower costs
  • Higher throughput
  • Minimal training required
  • Environmentally friendly
  • Eliminates ion suppression effects often associated with phospholipids
  • Tips offered to fit many formats: Tecan®, Hamilton®, INTEGRA, and Universal liquid handling platforms and pipettors.

Legal Information

Hamilton is a registered trademark of Hamilton Co.
HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany
Tecan is a registered trademark of Tecan Group AG

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(C)

Not applicable

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Questions

  1. What are the differences between the Hybrid DPX Tip, BioSPME Tip, and the ZipTip?

    1 answer

    1. DPX Tips are dispersive SPE (solid phase extraction) tips primarily used in small molecule applications. They facilitate sample preparation in the LCMS workflow by allowing automated and rapid extraction through simple pipetting up and down at each step. The HybridSPE sorbent inside is designed to remove phospholipids from biological samples, making these tips particularly suitable for clinical and other applications related to blood, serum, plasma, or any matrix where phospholipids are a concern. Additionally, DPX Tips with Supel Swift HLB material inside, targeting cleanup for clinical applications, will soon be available, with the initial application notes focusing on opioids in urine and hormones in serum.

      BioSPME SPEit Tips do not draw up any liquid. They consist of an SPME fiber affixed to a pipette tip, allowing for the extraction of small analytes of interest from the sample. However, they are noted to be less reproducible, and not recommended unless specifically required for free unbound small analytes. They have limited applicability.

      Ziptips are primarily used for large molecule applications and desalting of peptides

      Helpful?

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