Skip to Content
Merck
All Photos(1)

Documents

V2260

Sigma-Aldrich

Monoclonal Anti-V5-Peroxidase antibody produced in mouse

clone V5-10, purified immunoglobulin, lyophilized powder

Synonym(s):

Monoclonal Anti-V5

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

MDL number:
MFCD03457579
UNSPSC Code:
12352203
NACRES:
NA.56

biological source

mouse

Quality Level

200

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

V5-10, monoclonal

form

lyophilized powder

packaging

vial of 0.5 mL

concentration

5-11 mg/mL

technique(s)

western blot: 1:4,000-1:8,000

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

General description

The V5 tag is a 14-amino acid long peptide sequence (GKPIPNPLLGLDST) that is derived from an epitope on the P and V proteins of simian virus 5 (SV5), which belongs to the paramyxovirus family. Monoclonal Anti-V5 Peroxidase conjugate reacts with V5-tagged recombinant fusion proteins expressed in transfected mmmalian cells or produced by in vitro translation.

Specificity

Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins

Application

Suitable for immunoblotting.

Physical form

Lyophilized from a 0.01 M phosphate buffered saline solution, pH 7.4, containing 1% BSA and 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Reconstitute with 0.5 mL of distilled water.

Not finding the right product?  

Try our Product Selector Tool.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Panayotis C Theodoropoulos et al.
Nature chemical biology, 12(4), 218-225 (2016-02-02)
A hallmark of targeted cancer therapies is selective toxicity among cancer cell lines. We evaluated results from a viability screen of over 200,000 small molecules to identify two chemical series, oxalamides and benzothiazoles, that were selectively toxic at low nanomolar
Jordan N Ranum et al.
Nucleic acids research, 52(6), 3199-3212 (2024-02-26)
Productive infections by RNA viruses require faithful replication of the entire genome. Yet many RNA viruses also produce deletion-containing viral genomes (DelVGs), aberrant replication products with large internal deletions. DelVGs interfere with the replication of wild-type virus and their presence
Shuang Li et al.
iScience, 27(5), 109646-109646 (2024-04-19)
Most advanced colorectal cancer (CRC) patients cannot benefit from targeted therapy due to lack of actionable targets. By mining data from the DepMap, we identified FAM126B as a specific vulnerability in CRC cell lines exhibiting low FAM126A expression. Employing a
Steven F Baker et al.
Cell reports, 24(10), 2581-2588 (2018-09-06)
Adaptation of viruses to their hosts can result in specialization and a restricted host range. Species-specific polymorphisms in the influenza virus polymerase restrict its host range during transmission from birds to mammals. ANP32A was recently identified as a cellular co-factor
Vy Tran et al.
Nature microbiology, 5(12), 1490-1503 (2020-08-26)
Cells infected by influenza virus mount a large-scale antiviral response and most cells ultimately initiate cell-death pathways in an attempt to suppress viral replication. We performed a CRISPR-Cas9-knockout selection designed to identify host factors required for replication after viral entry.
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service