Urease from Canavalia ensiformis (Jack bean)

Name: Urease from <I>Canavalia ensiformis</I> (Jack bean)
Brand: SIGMA

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About This Item

CAS Number:

9002-13-5

UNSPSC Code:

12352204

eCl@ss:

32160410

EC Number:

232-656-0

NACRES:

NA.54

MDL number:

MFCD00070858

EC Number:

3.5.1.5 (BRENDA, IUBMB)

Biological source:

Canavalia ensiformis

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Properties

biological source

Canavalia ensiformis

Quality Segment

200

form

solid

mol wt

hexamer 545 kDa, trimer ~272 kDa

contains

~20% dithiothreitol

suitability

suitable for marker for electrophoresis (non-denaturing PAGE)

storage temp.

−20°C

Description

General description

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620

Urease is a multi-subunit nickel-dependent metalloenzyme. It is found abundantly in fungi, yeast, plants, and bacteria. It belongs to the superfamily of amidohydrolases and phosphotriesterases. Urease is also found in leguminous seeds.

Application

Urease from Canavalia ensiformis (Jack bean) has been used:

as a component of the molecular mass standard in electrophoresis
as a component of the standard in size-exclusion chromatography
as a reference in native-polyacrylamide gel electrophoresis (PAGE)

Biochem/physiol Actions

Urease is an essential enzyme of the nitrogen cycle. It plays a role in degrading urea into ammonia and carbamate. Urease provides a source of nitrogen for growth to various organisms. It also plays a major role in seed germination and seed chemical defense.