This product is stable at room temperature at least until the assigned retest date reported in the Certificate of Analysis. Products that, while considered stable, may be subject to degradation over time are assigned a periodic retest date to confirm quality. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/t2569#product-documentation
T2569
Trizma® hydrochloride solution
pH 7.8, BioPerformance Certified, 1 M, suitable for cell culture
Synonym(s):
Tris hydrochloride solution
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About This Item
NACRES:
NA.25
UNSPSC Code:
12161700
Pricing and availability is not currently available.
Quality Segment
grade
BioPerformance Certified, Molecular Biology
form
solution
concentration
1 M
technique(s)
cell culture | mammalian: suitable
impurities
DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm Heavy metals (as Pb)
pH
7.8
useful pH range
7.0-9.0
absorption
≤0.05 at 290 at 40%
General description
Tris(hydroxymethyl)aminomethane (Tris) is a routine buffer in biological sciences. The pH buffering range is between 7.0 -9.0. It displays negligible binding towards metal in solutions. Tris is used majorly in combination with ethylenediaminetetraacetic acid (EDTA), especially for electrophoretic studies. Its low ionic mobility finds usage in capillary electrochromatography as well.
A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.
Application
Trizma® hydrochloride solution has been used:
- as a component of lysis buffer for lysing human embryonic kidney (HEK) 293 cells
- in the 10X digoxigenin (DIG) deoxynucleoside triphosphate (dNTP) nick translation labeling buffer and in tris EDTA and sodium chloride (TEN) buffer[1]
- in the preparation of tris, acetate, ethylenediaminetetraacetic acid (EDTA) (1x TAE) buffer and as a component of 10X gel loading dye[2]
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
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This Item | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| grade BioPerformance Certified, Molecular Biology | grade BioPerformance Certified, Molecular Biology | grade BioPerformance Certified, Molecular Biology | grade BioPerformance Certified, Molecular Biology |
| concentration 1 M | concentration 1 M | concentration 1 M | concentration 2 M |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form solution | form solution | form solution | form solution |
| pH 7.8 | pH 7.6 | pH 9.0 | pH 7.95—8.05 |
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Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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