Skip to Content
Merck
All Photos(1)

Documents

SRP3068

Sigma-Aldrich

IGF-Binding Protein-5 human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

IBP-5

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
51111800
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≤0.3 μg/mL

mol wt

28.6 kDa

packaging

pkg of 25 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IGFBP5(3488)

General description

IGFBP5 (insulin-like growth factor-binding protein 5) belongs to the IGFBP family of proteins. IGFBPs are mainly required to regulate IGFs (insulin like growth factors) activities. The gene IGFBP5 is mapped to human chromosome 2q35.
Recombinant human IGF-BP5 is a 28.6kDa protein consisting of 253 amino acid residues.

Biochem/physiol Actions

IGFBP5 (insulin-like growth factor-binding protein 5) works via IGF (insulin like growth factor)-dependent and -independent mechanisms. It can function as a tumor suppressor as well as an oncogene. Changes in the activity of this gene are often observed in human cancers and metastatic tissues. During cancer development, IGFBP5 is involved in cell growth, migration and invasion. IGFBP5 is associated with breast cancer, osteosarcoma, head and neck squamous cell carcinoma, neuroblastoma, prostate cancer and malignant melanoma. It is upregulated in systemic sclerosis and idiopathic pulmonary fibrosis. In primary fibroblasts, IGFBP5 exhibits pro-fibrotic effects. It is strongly expressed in bones and is involved in osteogenic differentiation. Under in vitro conditions, IGFBP5 and its microRNA (microRNA-143-3p) are involved in muscle regeneration.

Sequence

MLGSFVHCEP CDEKALSMCP PSPLGCELVK EPGCGCCMTC ALAEGQSCGV YTERCAQGLR CLPRQDEEKP LHALLHGRGV CLNEKSYREQ VKIERDSREH EEPTTSEMAE ETYSPKIFRP KHTRISELKA EAVKKDRRKK LTQSKFVGGA ENTAHPRIIS APEMRQESEQ GPCRRHMEAS LQELKASPRM VPRAVYLPNC DRKGFYKRKQ CKPSRGRKRG ICWCVDKYGM KLPGMEYVDG DFQCHTFDSS NVS

Physical form

Lyophilized from 10 mM Sodium Citrate, pH 3.0.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Age-related changes in miR-143-3p:Igfbp5 interactions affect muscle regeneration.
Soriano-Arroquia A, et al.
Aging Cell, 15, 361-361 (2016)
IGFBP-5 Promotes Fibrosis Independently of Its Translocation to the Nucleus and Its Interaction with Nucleolin and IGF.
Su Y, et al.
PLoS ONE, 10, e0130546-e0130546 (2015)
Junyun Wang et al.
Oncotarget, 6(24), 20636-20649 (2015-05-27)
The insulin-like growth factor binding protein 5 (IGFBP5), which is often dysregulated in human cancers, plays a crucial role in carcinogenesis and cancer development. However, the function and underlying mechanism of IGFBP5 in tumor growth and metastasis has been elusive
Maya Ghoussaini et al.
Nature communications, 4, 4999-4999 (2014-09-24)
GWAS have identified a breast cancer susceptibility locus on 2q35. Here we report the fine mapping of this locus using data from 101,943 subjects from 50 case-control studies. We genotype 276 SNPs using the 'iCOGS' genotyping array and impute genotypes
Alison J Butt et al.
The Journal of biological chemistry, 278(32), 29676-29685 (2003-06-05)
The role of insulin-like growth factor-binding protein (IGFBP)-5 in human breast cancer cell growth is unclear. We determined the effects of IGFBP-5 expression on the growth of human breast cancer cell lines in vivo and in vitro. Expression of IGFBP-5

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service