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SAB4700290

Sigma-Aldrich

Monoclonal Anti-CD42b antibody produced in mouse

clone HIP1, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-GP1BA, Anti-GPIb α

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

HIP1, monoclonal

form

buffered aqueous solution

species reactivity

human, nonhuman primates

concentration

1 mg/mL

technique(s)

flow cytometry: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... GP1BA(2811)

General description

Cluster of differentiation 42b (CD42b), also known as, glycoprotein Ib platelet α subunit (GP1BA), is encoded by the gene mapped to human chromosome 17p13.2.
The antibody HIP1 reacts with CD42b (GPIb alpha), a 135-145 kDa membrane glycoprotein expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are composed in a disulfide linked heterodimer (CD42b/c; 160 kDa); CD42b/c forms a noncovalent complex with CD42a and CD42d.

Immunogen

Peripheral blood mononuclear cells of a patient suffering with CLL

Application

The reagent is designed for Flow Cytometry analysis. Suggested working dilution for Flow Cytometry is 2-8 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Biochem/physiol Actions

Cluster of differentiation 42b (CD42b) clustering promotes integrin αIIbβ3-dependent platelet aggregation, phagocytosis and rapid platelet clearance in the liver. The encoded protein acts as a platelet receptor for von Willebrand factor (VWF) and other ligands present in circulation. Mutation in the gene leads to autosomal recessive Bernard-Soulier syndrome. CD42b interacts with plasma hemoglobin (Hb) and enhances platelet activation and apoptosis in a concentration-dependent manner and also regulates platelet functions.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Solution in phosphate buffered saline, pH 7.4, with 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Inhibiting GPIb? shedding preserves post-transfusion recovery and hemostatic function of platelets after prolonged storage
Chen W
Arteriosclerosis, Thrombosis, and Vascular Biology, 36, 1821-1828 (2016)
Hemoglobin interaction with GP1b? induces platelet activation and apoptosis: a novel mechanism associated with intravascular hemolysis
Singhal R
Haematologica, 100, 1526-1533 (2015)
Glycoprotein Ib? clustering induces macrophage-mediated platelet clearance in the liver.
Yan R
Thrombosis and Haemostasis, 113, 107-117 (2015)
Genomic landscape of megakaryopoiesis and platelet function defects.
Elisa B, et al.
Blood, 127, 1249-1259 (2016)

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