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S9751

Sigma-Aldrich

Sulfatase from Helix pomatia

Type H-2, aqueous solution, ≥2,000 units/mL

Synonym(s):

Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase

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About This Item

CAS Number:
9016-17-5
Enzyme Commission number:
3.1.6.1 (BRENDA, IUBMB)
MDL number:
MFCD00132399
UNSPSC Code:
12352204
NACRES:
NA.54

type

Type H-2

form

aqueous solution

secondary activity

≥100,000 units/mL β-glucuronidase (at pH 5.0)

mol wt

~85 kDa by gel filtration

concentration

≥2,000 units/mL

shipped in

wet ice

storage temp.

2-8°C

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General description

Sequential action of sulfatase from Helix pomatia and β-O-glucosidase from Caldicellulosiruptor saccharolyticus on glucosinolates allows synthesis of thiohydroximates. Sulfatase from Helix pomatia hydrolyzes β-naphthyl sulfate much faster than α-naphthyl sulfate.

Application

Sulfatase from Helix pomatia is often used to remove sulfate groups from carbohydrates.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hour at pH 5.0 at 37 °C (30 min assay).

substrate

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Abigail M Tokheim et al.
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, 18(5), 537-540 (2005-12-08)
Often used to remove sulfate groups from carbohydrates, the regulatory properties of the aryl sulfatase from Helix pomatia remain little characterized. As many hydrolytic enzymes utilize exogenous metal ions in catalysis, the effect of various divalent metal ions on the
Tania Merinas-Amo et al.
Foods (Basel, Switzerland), 10(11) (2021-11-28)
Brassica rapa L. subsp. rapa (turnip greens), a traditionally consumed vegetable, is well-known due to its high content of glucosinolates, which are secondary metabolites with a positive biological activity for human health. Our hypothesis has been based on the relation
Fernando Cámara-Martos et al.
Foods (Basel, Switzerland), 11(3) (2022-02-16)
In northwest Spain and Portugal, there is a long tradition of cultivating B. rapa subsp. rapa to obtain turnip greens and turnip tops. Brassica rapa L. subsp. rapa (turnip greens and turnip tops) were grown under conventional and organic conditions
Jakub Kopycki et al.
Biotechnology letters, 33(5), 1039-1046 (2011-01-27)
Thiohydroximates comprise a diverse class of compounds important in both biological and industrial chemistry. Their syntheses are generally limited to simple alkyl and aryl compounds with few stereocenters and a narrow range of functional groups. We hypothesized that sequential action
G Pelsy et al.
Biotechnology and bioengineering, 25(4), 919-928 (1983-04-01)
It has been found that sulfatase from Helix pomatia hydrolyzes beta-naphthyl sulfate much faster than alpha-naphthyl sulfate; e.g., at pH 7.8, while the former is readily hydrolyzed, the latter undergoes no appreciable hydrolysis. Kinetic investigations of both enzymatic and acid
View All Related Papers

Protocols

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)

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