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Sodium Bicarbonate

Name: Sodium Bicarbonate
Brand: SIGMA

Solution (7.5%), sterile-filtered, suitable for cell culture

Synonym(s):

aqueous sodium bicarbonate

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About This Item

CAS Number:

144-55-8

UNSPSC Code:

12161702

PubChem Substance ID:

24899815

NACRES:

NA.75

MDL number:

MFCD00003528

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Properties

Product Name

Sodium bicarbonate solution, solution (7.5%), sterile-filtered, BioReagent, suitable for cell culture

sterility

sterile-filtered

Quality Segment

400

product line

BioReagent

form

solution (7.5%)

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

7.8-8.2

storage temp.

2-8°C

SMILES string

[Na+].OC([O-])=O

InChI

1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1

InChI key

UIIMBOGNXHQVGW-UHFFFAOYSA-M

Description

General description

Sodium bicarbonate (NaHCo₃) is a commonly used alkaline buffering agent(5) in laboratories. The addition of this buffer to the cell culture medium aids in maintaining the appropriate pH by using the carbon dioxide present in the incubator. In addition to its buffering properties, Sodium bicarbonate also acts as a source of sodium and aids in sodium gluconate synthesis during the fermentation process.

Application

Sodium bicarbonate solution has been used:

as a component of Hanks′ buffered salt solution (HBSS) and recording medium for culturing of tissue explants from rats or mice
as a component of Dulbecco′s modified Eagle′s medium (DMEM) to culture mice tissue explants
as a component of Minimum Essential Media (MEM) to study the cytotoxicity against human colorectal adenocarcinoma cells and mammary gland adenocarcinoma
to prepare collagen mix droplets for 3D collagen sprouting assay
to prepare collagen matrix for rat epidermal keratinocyte (REK) organotypic culture

Preparation Note

Prepared in cell culture grade water.

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This Item	S6014	1613655	13433
Sigma-Aldrich S8761 Sodium bicarbonate solution	Sigma-Aldrich S6014 Sodium bicarbonate	USP 1613655 Sodium bicarbonate	Sigma-Aldrich 13433 Sodium bicarbonate
description solution (7.5%), sterile-filtered, BioReagent, suitable for cell culture	description ACS reagent, ≥99.7%	description United States Pharmacopeia (USP) Reference Standard	description puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, E500, 99.0-100.5%, powder
technique(s) cell culture \| mammalian: suitable	technique(s) -	technique(s) -	technique(s) -
Quality Level 400	Quality Level 300	Quality Level -	Quality Level 200
product line BioReagent	product line -	product line -	product line -
form solution (7.5%)	form powder	form -	form powder
storage temp. 2-8°C	storage temp. -	storage temp. -	storage temp. -
pH 7.8-8.2	pH -	pH 8.3 (0.1 molfreshly prepared)	pH ≤8.6 (20 °C, 5%)

Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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