P8804
Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus
buffered aqueous glycerol solution, ≥1,000 units/mg protein (Lowry)
Synonym(s):
1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming, PI-PLC
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
232-638-2
MDL number:
Specific activity:
≥1,000 units/mg protein (Lowry)
Biological source:
Bacillus sp. (Bacillus cereus)
biological source
Bacillus sp. (Bacillus cereus)
Quality Segment
form
buffered aqueous glycerol solution
specific activity
≥1,000 units/mg protein (Lowry)
mol wt
28 kDa
foreign activity
Phopholipase C (lecithinase) ≤1 units/mg protein, Sphingomyelinase ≤40 units/mg protein
storage temp.
2-8°C
Gene Information
Bacillus cereus E33L ... BCZK3513(3026815)
General description
Phospholipase C (PI-PLC) from Bacillus cereus has irregular triosephosphate isomerase (TIM)-barrel structure with eight-standard parallel β barrel. It is a 28 kDa protein.
Application
Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used:
- in the hydrolysis of substrates p-nitrophenylphosphorylcholine (p-NPPC) and p-nitrophenylphosphorylphosphate (p-NPP)
- to cleave glycosylphosphatidylinositol (GPI) anchor of lynx1 protein and its detachment from plasma membrane
- to cleave immunolabeled HeLa cells
Biochem/physiol Actions
Used for the release of GPI anchored proteins from the membrane.
Phospholipase C (PI-PLC) releases diacylglycerol by cleaving the glycosylphosphatidylinositol. It has broad substrate specificity and its activity is influenced by metal ions and surfactants.
Physical form
Solution in 60% (v/v) glycerol containing 10 mM Tris-HCl, pH 8.0 and 10 mM EDTA
Analysis Note
Acetylcholinesterase is measured according to Ellman, et al.
Other Notes
One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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