P6774
Phosphatase, Alkaline from bovine intestinal mucosa
buffered aqueous solution, ≥2,000 DEA units/mg protein
Synonym(s):
Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
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About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
42010105
NACRES:
NA.54
EC Number:
MDL number:
Specific activity:
≥2,000 DEA units/mg protein
Biological source:
bovine intestinal mucosa
Concentration:
5-20 mg/mL
Pricing and availability is not currently available.
biological source
bovine intestinal mucosa
Quality Segment
form
buffered aqueous solution
specific activity
≥2,000 DEA units/mg protein
mol wt
dimer ~160 kDa
concentration
5-20 mg/mL
technique(s)
protein detection: suitable
storage temp.
2-8°C
General description
Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.
Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.2 The enzyme requires zinc, and magnesium or calcium divalent ions for activity.
Application
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It is routinely used to dephosphorylate proteins, such as casein, and nucleic acids. It may be used for protein labeling when high sensitivity is required. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase.. Product P6774 has been used during preparation of brain lysate.
The enzyme from Sigma has been used in the preparation of AP-BGG (alkaline phosphatase-bovine gamma globulin) conjugates. It has been conjugated to sheep anti-rabbit IgG and sheep anti-rabbit IgM in immunological assays. It has also been used to prepare double-enzyme conjugates with HRP for the simultaneous detection of three different intracellular Ig determinants in a single tissue section.
Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..
High specific activity grade recommended for antibody and protein conjugation.
Biochem/physiol Actions
The enzyme is a glycoprotein containing approximately 12% carbohydrate (6% hexoses and 6% other neutral sugars). Each molecule of alkaline phosphatase contains four zinc atoms and four disulfide bridges. Maximal activity with alkaline phosphatase is achieved in the presence of magnesium. It catalyzes the hydrolysis of phosphate monoesters such as p-nitrophenyl phosphate, phenyl phosphate, phenolphthalein phosphate, α-glycerol phosphate, β-glycerol phosphate, 2-phosphorylglycerate, triosephosphate, glucose-6-phosphate, glucose 1-phosphate, fructose 1-phosphate, fructose 6-phosphate, adenosine 5-phosphate adenosine 3-phosphate, phosphoenolpyruvate, and β-nicotinamide adenine dinucleotide phosphate. Arsenate, cysteine, iodine, inorganic phosphate, pyrophosphate, diisopropyl phosphate, triphenylphosphate, diisopropyl fluorophosphate, and L-phenylalanine are some of the strong inhibitors of alkaline phosphatase.
Alkaline phosphatase, from bovine intestinal mucosa, is most stable in the pH range 7.5-9.5. The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols and it requires zinc, and magnesium or calcium divalent ions for activity.
The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids.
Physical form
Solution in 3.0 M NaCl containing 5 mM MgCl2, 0.2 mM ZnCl2, and 30 mM triethanolamine, pH 7.6
Preparation Note
Affinity purified
Analysis Note
Package sizes are based on DEA units
Protein determined by biuret.
Other Notes
One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. (One glycine unit is equivalent to ~3 DEA units)
1 of 1
This Item | |||
|---|---|---|---|
| description buffered aqueous solution, ≥2,000 DEA units/mg protein | description buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer) | description lyophilized powder, 30-60 units/mg protein (in glycine buffer) | description ≥10 units/mg solid (in glycine buffer) |
| specific activity ≥2,000 DEA units/mg protein | specific activity 20-50 units/mg protein (in glycine buffer) | specific activity 30-60 units/mg protein (in glycine buffer) | specific activity ≥10 units/mg solid (in glycine buffer) |
| biological source bovine intestinal mucosa | biological source Escherichia coli | biological source - | biological source human placenta |
| form buffered aqueous solution | form buffered aqueous glycerol solution | form lyophilized powder | form - |
| concentration 5-20 mg/mL | concentration - | concentration - | concentration - |
| mol wt dimer ~160 kDa | mol wt - | mol wt - | mol wt - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
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Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
WGK
WGK 1
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