This product is a 2X crystalized preparation. The minimum protein content is 80%, which is generally associated with purity. However, the purity of this material has not been determined.
P4762
Papain from papaya latex
lyophilized powder, ≥10 units/mg protein
Papain from papaya latex
Synonym(s):
Papainase
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About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
42030311
EC Number:
232-627-2
NACRES:
NA.54
MDL number:
Specific activity:
≥10 units/mg protein
Biological source:
papaya (latex)
Pricing and availability is not currently available.
biological source
papaya (latex)
Quality Segment
form
lyophilized powder
specific activity
≥10 units/mg protein
mol wt
23.4 kDa
technique(s)
cell culture | mammalian: suitable, single cell analysis: suitable
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
General description
Papain is a cysteine protease, found in Carica papaya. It is composed of a single polypeptide chain containing three disulfide bridges and a sulfhydryl group.
Application
Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.
Papain from papaya latex has been used:
- to digest carotid artery smooth muscle cells[1]
- in dissecting solutions
- to produce Fab fragments of antibodies
- for cell dissociation since it has been shown to be more effective and less damaging with certain tissues[2][3]
- in the enzyme cocktail for dissociating human fetal retinal tissue
- to digest chondrocytes/scaffold cultures for total DNA quantification for cell proliferation
- as a supplement in neurobasal medium to produce retinal cell suspensions[4]
Biochem/physiol Actions
A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.
Papain with wide specificity, cleaves peptide bonds of basic amino acids, leucine or glycine. It also hydrolyzes esters and amides. Papain is considered to digest protein substrates more broadly than the pancreatic proteases. Papain is commonly used in cell isolation procedures as it is highly efficient and less harmful than other proteases on certain tissues. The use of papain is known to yield more viable smooth muscle cells, retaining the cell′s sensitivity to stimulants. Papain papaya latex has antifungal activity against C. albicans and is a cysteine protease.
- The pH optimum is 6.0-7.0
- Papain hydrolyzes esters and amides
Packaging
Package size based on protein content
Physical form
Lyophilized powder containing sodium acetate
Preparation Note
2× Crystallized
Other Notes
One unit will hydrolyze 1.0 micromole of N-alpha-benzoyl-L-arginine ethyl ester (BAEE) per minute at pH 6.2 at 25 deg C.
View more information on papain at www.sigma-aldrich.com/enzymeexplorer.
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This Item | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) - |
| biological source papaya (latex) | biological source papaya (latex) | biological source papaya | biological source - |
| specific activity ≥10 units/mg protein | specific activity ≥8.0 units/mg protein | specific activity ≥10 U/mg protein (~25 mg/ml) | specific activity - |
| form lyophilized powder | form lyophilized powder | form solution | form solid |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) - | application(s) USP Biologics |
| mol wt 23.4 kDa | mol wt 23.4 kDa | mol wt - | mol wt - |
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Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Is the product tested for endotoxin?
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This product is not tested for endotoxins. Currently, an endotoxin-tested version of Papain is not available.
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