N3393

Naphthol Blue Black

BioReagent, suitable for electrophoresis

• Synonym(s): Acid Black 1, Amido Black 10B, Buffalo Black NBR
• Empirical Formula (Hill Notation): C₂₂H₁₄N₆Na₂O₉S₂
• CAS Number: 1064-48-8
• Molecular Weight: 616.49
• EC Number: 213-903-1
• UNSPSC Code: 41105322
• NACRES: NA.32
• PubChem Substance ID: 24897642
• MDL number: MFCD00004017
• Beilstein/REAXYS Number: 5374263
• Colour Index Number: 20470

Properties

• product line: BioReagent
• Quality Segment: 200
• form: powder
• composition: Dye content, ~85%
• technique(s): protein electrophoresis: suitable, protein staining: suitable
• impurities: ≤10% water
• suitability: suitable for electrophoresis
• SMILES string: [Na+].[Na+].Nc1c(\N=N\c2ccc(cc2)[N+]([O-])=O)c(cc3cc(c(\N=N\c4ccccc4)c(O)c13)S([O-])(=O)=O)S([O-])(=O)=O
• InChI: 1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;
• InChI key: AOMZHDJXSYHPKS-DROYEMJCSA-L

Description

Application

Naphthol Blue Black has been used for the staining of western blot membrane for detection of all protein that are transferred to the membrane.

Biochem/physiol Actions

Naphthol blue black dye can be used to stain proteins on polyacrylamide gels, agarose gels and nitrocellulose membranes. After electrophoresis, fixing the proteins in the gel is recommended. The gel can then be stained with 0.1% Naphthol Blue Black in 7% (v/v) acetic acid for at least 2 hr and destained with a soluion of 7% (v/v) acetic acid. Detection sensitivity is approx. 20% that of Coomassie Blue R.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Codes: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1B
• Storage Class: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): dust mask type N95 (US), Eyeshields, Gloves