MSSAFE

Protease and Phosphatase Inhibitor Cocktail

MS-SAFE, powder, Protease inhibitors: serine, cysteine and aspartic proteases and metalloproteases. Phosphatase inhibitors: tyrosine, serine/threonine, acid and alkaline phosphatases.

• Synonym(s): Mass Spectrometry Safe Protease and Phosphatase Inhibitor Cocktail
• UNSPSC Code: 12352200
• NACRES: NA.77
• Form: powder
• Solubility: water: 0.1 g/mL, clear, colorless
• Storage temp.: 2-8°C

Properties

• Product Name: MS-SAFE Protease and Phosphatase Inhibitor,
• description: for use with mammalian cell and tissue extract, lyophilized powder
• Quality Segment: 200
• form: powder
• solubility: water: 0.1 g/mL, clear, colorless
• storage temp.: 2-8°C

Description

General description

Mass spectrometry (MS) compatible protease inhibitor cocktail and phosphatase inhibitor cocktail, with broad specificity for the inhibition of:

• Serine, cysteine and aspartic proteases, and metalloproteases
• Tyrosine, serine/threonine, acid and alkaline phosphatases

All inhibitors and fillers were chosen so as not to interfere with LC-MS analyses. Any inhibitors capable of covalent, irreversible protein modification were avoided. This cocktail is EDTA-free. All inhibitors were selected to allow facile downstream sample processing, such as immobilized metal affinity chromatography (IMAC) for His-tagged protein purification and, uniquely, phosphopeptide enrichment.

Application

Tested in mammalian cell lysates and liver tissue extracts. Designed for use in samples to be analyzed by mass spectrometry.

MS-SAFE Protease and Phosphatase Inhibitor has been used to measure ribosomal protein S6 kinase (S6K) activity.

Biochem/physiol Actions

Protease inhibitors: serine, cysteine and aspartic proteases, and metalloproteases
Phosphatase inhibitors: tyrosine, serine/threonine, acid and alkaline phosphatases

Features and Benefits

Comprehensive protease and phosphatase inhibitor cocktail for mass spectrometry analysis

Compatible with downstream sample processing such as His-tagged protein purification and phosphopeptide enrichment

Allows accurate measurement of protein activity and identification of phosphorylation sites.

Physical form

Lyophilized powder that is water-soluble

Preparation Note

One vial makes 20 mL of 1× inhibitor cocktail working solution, using either water or extraction/lysis buffer. Alternatively, a 10× concentrated solution may be prepared by adding 2 mL of water or extraction/lysis buffer. This 10× solution may then be diluted 10-fold into extraction/lysis buffer as needed for a 1× working solution.

Other Notes

Protease inhibitors:

• Bestatin hydrochloride
• Leupeptin
• Phosphoramidon disodium salt
• Pepstatin A
• Elastatinal
• Aprotinin
• Nafamostat mesylate
• Antipain

Phosphatase inhibitors

• Okadaic acid
• Sodium fluoride
• Sodium orthovanadate
• Bromotetramisole oxalate

Fillers

• β-lactose
• DL-leucine

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H302,H315,H319
• pcodes: P264 - P270 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable