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Key Documents

MAK318

Sigma-Aldrich

α−Mannosidase Assay Kit

sufficient for 100 colorimetric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

detection method

colorimetric

relevant disease(s)

neurological disorders

storage temp.

2-8°C

General description

α-Mannosidase (AMA) is an enzyme which catalyzes the cleavage of the alpha form of mannose. α-Mannosidase assists in the breakdown of complex sugars from glycoproteins in the lysosome. Defective AMA or deficient AMA activity causes α-mannosidosis and leads to deterioration of the central nervous system in children.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for determining α-Mannosidase (AMA) activity in a variety of biological samples such as plasma, serum, tissue, and cell culture media.

Principle

The α-Mannosidase Activity Assay Kit provides a simple and high-throughput adaptable assay for determining α-Mannosidase activity in a variety of biological samples such as plasma, serum, tissue, and cell culture media. In this assay 4-nitrophenol is cleaved from a synthetic substrate. Nitrophenol becomes intensely colored with the addition of the stop reagent, producing a colorimetric (405 nm) result, directly proportional to the enzyme activity in the sample.
One unit of AMA is the amount of enzyme that will convert 1.0 μmole of 4-Nitrophenyl-α-D-mannopyranoside to 4-Nitrophenol and α-D-Mannose per minute at 25 °C and pH 4.5. The kit has a linear detection range of 1–250 unit/L for a 10 minute reaction in a 96 well format.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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