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M5904

Mineral oil

BioReagent, Molecular Biology, light oil

Synonym(s):

light mineral oil

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About This Item

CAS Number:
UNSPSC Code:
12181504
NACRES:
NA.52
EC Number:
232-455-8
MDL number:
Grade:
Molecular Biology
Technique(s):
PCR: suitable
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grade

Molecular Biology

Quality Segment

product line

BioReagent

form

light oil

technique(s)

PCR: suitable

color

colorless

refractive index

n20/D 1.467 (lit.)

density

0.84 g/mL at 25 °C (lit.)

suitability

suitable for overlaying aqueous reactions and centrifuge gradients

foreign activity

DNase, RNase, Protease, none detected

storage temp.

room temp

InChI key

AEOVEGJBKQQFOP-DDVLFWKVSA-L

General description

Mineral oils are usually seen as a mixture of liquid hydrocarbons. It is derived from crude oil by distillation and refining.

Application

Mineral oil has been used:
  • to suspend the gels for capillary contraction assay
  • in polymerase chain reaction (PCR) reactions mixture to form a vapor barrier in order to prevent the evaporation of the reaction at elevated temperatures
  • as a continuous phase to prevent droplet coalescence in emulsification system

Mineral oil is suitable for use as an overlay to control evaporation and cross contamination of samples in a variety of molecular biology applications.[1][2][3]

Biochem/physiol Actions

Mineral oil delivers a better insulation strength, when compared to air. It enhances the elimination of heat from the windings, when used in a power transformer.

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This Item
M5310M3516330760
technique(s)

PCR: suitable

technique(s)

cell culture | embryo: suitable

technique(s)

PCR: suitable

technique(s)

-

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

grade

Molecular Biology

grade

-

grade

-

grade

-

form

light oil

form

light oil

form

light oil

form

liquid (colorless)

density

0.84 g/mL at 25 °C (lit.)

density

0.82-0.88 g/mL at 25 °C

density

0.84 g/mL at 25 °C (lit.)

density

0.862 g/mL at 25 °C

refractive index

n20/D 1.467 (lit.)

refractive index

n20/D 1.467 (lit.)

refractive index

n20/D 1.467 (lit.)

refractive index

n20/D 1.467 (lit.)


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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

No data available

flash_point_c

No data available

ppe

Eyeshields, Gloves



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Questions

1–4 of 4 Questions  
  1. What is the Specific Heat Capacity of this product?

    1 answer
    1. For catalog number M5904, we have not determined the heat capacity value. Here is the link for our SDS:
      https://www.sigmaaldrich.com/US/en/sds/sigma/m5904?userType=anonymous

      Helpful?

  2. Is this mineral oil suitable for microaerobic or anaerobic cultures of E. coli?

    1 answer
    1. This product has not been tested for cell culture use. However, in the literature, it is known to work with aerobic and anaerobic cell cultures.

      Helpful?

  3. Hello, I´m looking for a mineral oil for sulfur pattern production. The COA of this product says "pass" for sufurous compounds, what does this mean?

    1 answer
    1. The production and preparation of this product are considered proprietary. Currently, there is no product option available that is tested for the presence of sulfur.

      Helpful?

  4. Esiste una data di scadenza per il prodotto in questione?

    1 answer
    1. The product specification sheet for this product contains a recommended retest date period of roughly four years after the quality release date. A recommended retest date is the period of time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications. Please view more information here: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/190/408/product-dating-information-ms.pdf.

      Helpful?

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