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Minimum Essential Medium Eagle

Name: Minimum Essential Medium Eagle
Brand: SIGMA

with Earle′s salts and non-essential amino acids, without L-glutamine, phenol red and sodium bicarbonate, Modified, powder, suitable for cell culture

Synonym(s):

EMEM, MEM

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About This Item

NACRES:

NA.75

UNSPSC Code:

12352207

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Properties

Quality Segment

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

Earle’s salts (5% CO₂): yes
L-glutamine: no
HEPES: no
NaHCO₃: no
sodium pyruvate: no
phenol red: no

shipped in

ambient

storage temp.

2-8°C

Description

General description

Minimum Essential Medium Eagle contains 19 amino acids. The essential amino acids and the non-essential amino acids: L-ala, L-asn, L-asp, L-glu, L-gly, L-pro, and L-ser. This medium is preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium. It is suitable for a wide variety of cells grown in monolayers.

Application

Minimum Essential Medium Eagle has been used:

to culture endometrial adenocarcinoma Ishikawa cells for bisphenol studies
to culture MCF-7 cells for experimental treatments with hormone
to maintain zebrafish pineal glands for action spectrum analysis

Features and Benefits

Contains high concentrations of amino acids so the medium resembles the protein composition of cultured mammalian cells

Preparation Note

Formulated to contain 9.3 grams of powder per liter of medium.

Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate.

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This Item	M4526	M7278	M8042
Sigma-Aldrich M3024 Minimum Essential Medium Eagle	Sigma-Aldrich M4526 Minimum Essential Medium Eagle	Sigma-Aldrich M7278 Minimum Essential Medium Eagle	Sigma-Aldrich M8042 Minimum Essential Medium Eagle
description with Earle′s salts and non-essential amino acids, without L-glutamine, phenol red and sodium bicarbonate, Modified, powder, suitable for cell culture	description Alpha Modification, with sodium bicarbonate, without L-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture	description HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture	description Alpha Modification, with ribonucleosides, deoxyribonucleosides and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
form powder	form liquid	form liquid	form liquid
components Earle’s salts (5% CO₂): yes L-glutamine: no HEPES: no NaHCO₃: no sodium pyruvate: no phenol red: no	components Earle’s salts (5% CO₂): yes sodium pyruvate: yes glucose: yes L-glutamine: no HEPES: no Hanks’ salts (2% CO₂): no phenol red: yes NaHCO₃: yes stable glutamine: no	components L-glutamine: no phenol red: yes HEPES: 25 mM sodium pyruvate: no Earle’s salts (5% CO₂): yes NaHCO₃: yes	components Hanks’ salts (2% CO₂): no glucose: yes sodium pyruvate: yes phenol red: yes HEPES: no L-glutamine: no stable glutamine: no Earle’s salts (5% CO₂): yes NaHCO₃: yes
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
Quality Level 500	Quality Level 400	Quality Level 400	Quality Level 400

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Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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