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L9545

Sigma-Aldrich

Leukemia Inhibitory Factor human, animal component free

recombinant, expressed in rice, 500 μg/mL

Synonym(s):

Leukemia Inhibitory Factor human, LIF, rLIF

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About This Item

MDL number:
MFCD00163950
UNSPSC Code:
12352202
NACRES:
NA.32

recombinant

expressed in rice

Quality Level

200

Assay

>95% (SDS-PAGE)

potency

>1 x 108 units/mg ED<sub>50</sub>

concentration

500 μg/mL

technique(s)

cell culture | mammalian: suitable

impurities

<0.05 EU/μg endotoxin

UniProt accession no.

P15018

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... LIF(3976)

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Biochem/physiol Actions

Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein originally described to inhibit the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Other activities were later identified to LIF, which is known by a variety of synonyms, including DIF, D-factor, DIA, DRF, CNDF, HILDA, HSF-III, and MLPLI. Human LIF exerts its actions through a receptor comprising a 190 kDa LIF-binding α-chain (130 kDa, mouse) and a 130 kDa signal-transducing β-chain (gp130), which is shared with CNTF, OSM, L-6 and IL-11. As such, it is a member of the gp130 family of the cytokine receptor superfamily. LIF receptors have been identified on several cells, including monocytes, liver, placenta and embryonic stem cells. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, depending on the source, but absence of glycosylation appears not to affect its biological activity. A single gene encodes LIF, which is secreted as a single chain glycoprotein containing 180 amino acids for human or mouse with a conserved disulfide bond. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells.

Physical form

0.2 μm filtered solution at 500 μg/mL in buffered saline with 0.02% TWEEN®20

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Bradford A Youngblood et al.
Journal of biotechnology, 172, 67-72 (2014-01-02)
Embryonic and induced pluripotent stem cells have the ability to differentiate into any somatic cell type, and thus have potential to treat a number of diseases that are currently incurable. Application of these cells for clinical or industrial uses would
Takayuki Nakagomi et al.
Stem cells (Dayton, Ohio), 33(6), 1962-1974 (2015-02-20)
Brain vascular pericytes (PCs) are a key component of the blood-brain barrier (BBB)/neurovascular unit, along with neural and endothelial cells. Besides their crucial role in maintaining the BBB, increasing evidence shows that PCs have multipotential stem cell activity. However, their
Xiaoying Wang et al.
PloS one, 9(8), e106110-e106110 (2014-08-29)
Sertoli cells constitute the structural framework in testis and provide an immune-privileged environment for germ cells. Induced pluripotent stem cells (iPS cells) resemble embryonic stem cells (ES cells) and are generated from somatic cells by expression of specific reprogramming transcription
Tsuyoshi Tsukada et al.
PloS one, 10(6), e0129011-e0129011 (2015-06-05)
Recent studies in rodents suggest that maternal immune activation (MIA) by viral infection is associated with schizophrenia and autism in offspring. Although maternal IL-6 is though t to be a possible mediator relating MIA induced these neuropsychiatric disorders, the mechanism
Yufang Ma et al.
Neuro-oncology, 17(4), 516-525 (2014-09-14)
RhoB is a member of the Rho small GTPase family that regulates cytoskeletal dynamics and vesicle trafficking. The RhoB homologs, RhoA and RhoC, have been shown to promote cancer progression and metastasis. In contrast, the functions of RhoB in human
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Articles

Leukemia Inhibitory Factor (LIF) Protein

Leukemia Inhibitory Factor (LIF) is a stem cell growth factor used for the in vitro culture of pluripotent mouse embryonic stem cells (ES cells).

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