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Lectin from Triticum vulgaris (wheat)

Name: Lectin from <I>Triticum vulgaris </I>(wheat)
Brand: SIGMA

biotin conjugate, lyophilized powder

Synonym(s):

WGA, Wheat germ agglutinin

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About This Item

UNSPSC Code:

12352202

NACRES:

NA.32

eCl@ss:

34058011

Form:

lyophilized powder

Pricing and availability is not currently available.

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Properties

conjugate

biotin conjugate

Quality Segment

200

form

lyophilized powder

composition

Protein, ≥80% E1%/280

extent of labeling

2-4 mol biotin per mol protein

storage temp.

2-8°C

Description

General description

Wheat germ lectin (WGA) from Triticum vulgaris exists as a homodimer and has multiple N-acetyl glucosamine (GlcNAc) binding sites. It is resistant to protein denaturation agents and is used for the detection of O-GlcNAc proteins. WGA binding to N-glycolylneuraminic acid (Neu5Ac) may modulate pro-inflammatory immune response.

Application

Lectin from Triticum vulgaris (wheat) has been used:

in lectin binding assay of samples from intrauterine stages of filarial parasites
in the histological studies of mice myocytes
for the generation bead-biotin-WGA complex with Dynabeads^® M-280 Streptavidin magnetic beads for lectin magnetic separation of Toxoplasma gondii oocytes from water samples

Biochem/physiol Actions

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Packaging

Package size based on protein content.

Physical form

Contains sodium citrate

Preparation Note

Conjugates are prepared from affinity purified lectin.

Legal Information

Dynabeads is a registered trademark of Invitrogen Dynal AS Corp

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This Item	L0636	L9640	L1882
Sigma-Aldrich L5142 Lectin from Triticum vulgaris (wheat)	Sigma-Aldrich L0636 Lectin from Triticum vulgaris (wheat)	Sigma-Aldrich L9640 Lectin from Triticum vulgaris (wheat)	Sigma-Aldrich L1882 Lectin from Triticum vulgaris (wheat)
description biotin conjugate, lyophilized powder	description BioReagent, suitable for cell culture, lyophilized powder	description lyophilized powder	description agarose conjugate, saline suspension
form lyophilized powder	form lyophilized powder	form lyophilized powder	form saline suspension
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
Quality Level 200	Quality Level 200	Quality Level 300	Quality Level 200
conjugate biotin conjugate	conjugate -	conjugate -	conjugate agarose conjugate
composition Protein, ≥80% E1%/280	composition -	composition Protein, 100% biuret	composition -
extent of labeling 2-4 mol biotin per mol protein	extent of labeling -	extent of labeling -	extent of labeling 5-10 mg per mL

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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