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Key Documents

L4395

Sigma-Aldrich

Liposome Kit: Lipid mixtures for the preparation of liposomes

lyophilized powder

Synonym(s):

cholesterol, L-α-phosphatidylcholine and stearylamine kit

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About This Item

EC Number:
204-695-3
UNSPSC Code:
12352211
NACRES:
NA.25
Pricing and availability is not currently available.

biological source

egg yolk

Quality Level

200

form

lyophilized powder

composition

Cholesterol, 9 μmol/package
L-α-Phosphatidylcholine (egg yolk), 63 μmol/package
Stearylamine, 18 μmol/package

functional group

carboxylic acid

shipped in

ambient

storage temp.

−20°C

Related Categories

Application

Liposome Kit has been used for the preparation of liposomes.[1][2][3]

Biochem/physiol Actions

Liposome Kit: Lipid mixtures for the preparation of liposomes contains cholesterol; L-α-phosphatidylcholine and stearylamine. Liposome kit may be used to prepare cationic (positively charge) liposomes for incorporation of materials into cells.
Liposomes can be used for different applications such as drug delivery, diagnostics, membrane models, adjuvants for vaccination and others. This lipid mixture can be used to encapsulate a broad spectrum of hydrophilic and amphipathic molecules of low, medium, and high molecular weight (including peptides, proteins, and oligo- and polynucleotides).

related product

Product No.
Description
Pricing

Pictograms

CorrosionEnvironment
GHS05,GHS09

Signal Word

Danger

Hazard Statements

H318,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000418690
0000404712
0000404712
0000418690
0000408426

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Abstracts of the Congress of the European Association of Nuclear Medicine. 25-29 August 2001, Napoli, Italy.
European journal of nuclear medicine, 28(8), 961-1298 (2001-09-06)
Evaluation of intracellular and extracellular trehalose as a cryoprotectant of stem cells obtained from umbilical cord blood
Motta JPR, et al.
Cryobiology, 68(3), 343-348 (2014)
S Palakurthi et al.
International journal of pharmaceutics, 290(1-2), 55-62 (2005-01-25)
Conventional lipid microspheres (LM) were prepared using soybean oil and lipid at a 5.5:1 weight ratio with lipid phase consisting of PC (phosphatidyl choline):CH (cholesterol) (1:0.5) by molar ratio. The average diameter of the particles was 150 nm. Long-circulating microspheres
Abd Kakhar Umar et al.
Molecules (Basel, Switzerland), 26(17) (2021-09-11)
Human epidermal growth factor (hEGF) has been known to have excellent wound-healing activity. However, direct application to the wound area can lead to low hEGF bioavailability due to protease enzymes or endocytosis. The use of liposomes as coatings and carriers
Preparation and pharmacodynamic evaluation of liposomes of indomethacin.
Srinath P, Vyas SP, Diwan PV.
Drug Devel. Ind. Pharm., 26, 313-321 (2000)
View All Related Papers

Questions

1–7 of 7 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  4. I'm thinking of purchasing Product L4395, Liposome Kit.  Why would one use positive vs. negative liposomes?

    1 answer

    1. Analogous to poly-L-lysine, one may need positively charged agents to deliver drugs to cells, hence positive liposomes.

      Helpful?

  5. I have purchased Product L4395, Liposome Kit.  Is there a difference between positive and negative liposomes?

    1 answer

    1. The choice is dependent upon what is being encapsulated and how it is being used.  A good reference discussing this question is Ogiso, T., et al., Effect of positively and negatively charged liposomes on skin permeation of drugs. J. Drug Target, 9(1), 49-59 (2001).

      Helpful?

  6. How is Product L4395, Liposome Kit, used?

    1 answer

    1. The Liposomes of a large multilamellar structure type are the most widely used. They can be prepared using Sigma liposome mixtures by concentrating an organic solvent solution of lipids to a thin film on the walls of a container such as by rotary evaporation or by drying in glass with a stream of nitrogen gas. Then, under appropriate conditions, an aqueous solution is added to the thin film and with agitation multilamellar vesicles should form spontaneously with little effort. Large or small unilamellar structured liposomes can also be prepared by varying procedure or conditions. Please see the product insert for Product No. L4031 (the link below) for a step by step protocol that will work for this product also.

      Helpful?

  7. How do I prepare the Product L4395, Liposome Kit liposomes?

    1 answer

    1. Refer to the product information sheet (under Documents, above) for L4031 for preparation information.

      Helpful?

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