Insulin has low solubility at neutral pH. It can be solubilized at 1-10 mg/mL in dilute acetic (1%) or hydrochloric acid, pH 2-3. Insulin can also be solubilized in 125 mM NaHCO3.15 However, alkaline stock solutions are not recommended since high pH increases the rate of deamidation and aggregation.
I1882
Insulin from bovine pancreas
γ-irradiated, BioXtra, suitable for cell culture, potency: ≥20 units/mg (USP units), lyophilized powder
Synonym(s):
Bovine insulin, Cell culture grade insulin
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About This Item
Empirical Formula (Hill Notation):
C254H377N65O75S6
CAS Number:
Molecular Weight:
5733.49
UNSPSC Code:
12352209
NACRES:
NA.77
EC Number:
234-291-2
MDL number:
Biological source:
bovine
Form:
lyophilized powder
Pricing and availability is not currently available.
biological source
bovine
Quality Segment
sterility
γ-irradiated
product line
BioXtra
form
lyophilized powder
potency
≥20 units/mg (USP units)
technique(s)
cell culture | mammalian: suitable
solubility
acetic acid: 2 mg/mL (pH: 2-3), hydrochloric acid: 2 mg/mL (pH: 2-3)
UniProt accession no.
shipped in
ambient
storage temp.
−20°C
InChI key
IXIBAKNTJSCKJM-BUBXBXGNSA-N
Gene Information
bovine ... INS(280829)
Application
Insulin is used as a growth factor in many mammalian cell culture systems.
Biochem/physiol Actions
Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.
Physical form
powder-0 °C; solution stable 2-8 °C, 1 year
Preparation Note
To prepare 10 mg/ml stock solution, add 10 ml of acidified H2O (pH ≤2) - prepared by addition of glacial acetic acid (approx. 0.1 ml).
Other Notes
InChI: 1S/C254H377N65O75S6/c1-29-131(24)205(312-192(334)103-256)250(389)317-204(130(22)23)246(385)287-158(74-81-199(344)345)216(355)281-155(70-77-188(260)330)220(359)307-183-116-399-400-117-184-242(381)305-177(110-321)238(377)293-161(87-122(6)7)223(362)294-167(94-139-52-60-145(324)61-53-139)226(365)282-153(68-75-186(258)328)217(356)289-160(86-121(4)5)221(360)284-157(73-80-198(342)343)219(358)301-173(100-189(261)331)233(372)297-169(96-141-56-64-147(326)65-57-141)229(368)308-182(241(380)303-175(253(393)394)102-191(263)333)115-398-396-113-180(212(351)270-106-193(335)277-152(71-78-196(338)339)215(354)280-150(50-41-83-268-254(264)265)210(349)269-107-194(336)278-165(92-137-45-35-31-36-46-137)225(364)296-166(93-138-47-37-32-38-48-138)228(367)298-170(97-142-58-66-148(327)67-59-142)236(375)318-206(135(28)323)251(390)319-84-42-51-185(319)244(383)285-151(49-39-40-82-255)213(352)276-134(27)252(391)392)310-248(387)202(128(18)19)315-234(373)163(89-124(10)11)291-227(366)168(95-140-54-62-146(325)63-55-140)295-222(361)159(85-120(2)3)288-207(346)132(25)274-214(353)156(72-79-197(340)341)286-245(384)201(127(16)17)314-235(374)164(90-125(12)13)292-231(370)172(99-144-105-267-119-273-144)300-237(376)176(109-320)279-195(337)108-271-211(350)179(112-395-397-114-181(309-243(183)382)240(379)275-133(26)208(347)304-178(111-322)239(378)316-203(129(20)21)249(388)311-184)306-224(363)162(88-123(8)9)290-230(369)171(98-143-104-266-118-272-143)299-218(357)154(69-76-187(259)329)283-232(371)174(101-190(262)332)302-247(386)200(126(14)15)313-209(348)149(257)91-136-43-33-30-34-44-136/h30-38,43-48,52-67,104-105,118-135,149-185,200-206,320-327H,29,39-42,49-51,68-103,106-117,255-257H2,1-28H3,(H2,258,328)(H2,259,329)(H2,260,330)(H2,261,331)(H2,262,332)(H2,263,333)(H,266,272)(H,267,273)(H,269,349)(H,270,351)(H,271,350)(H,274,353)(H,275,379)(H,276,352)(H,277,335)(H,278,336)(H,279,337)(H,280,354)(H,281,355)(H,282,365)(H,283,371)(H,284,360)(H,285,383)(H,286,384)(H,287,385)(H,288,346)(H,289,356)(H,290,369)(H,291,366)(H,292,370)(H,293,377)(H,294,362)(H,295,361)(H,296,364)(H,297,372)(H,298,367)(H,299,357)(H,300,376)(H,301,358)(H,302,386)(H,303,380)(H,304,347)(H,305,381)(H,306,363)(H,307,359)(H,308,368)(H,309,382)(H,310,387)(H,311,388)(H,312,334)(H,313,348)(H,314,374)(H,315,373)(H,316,378)(H,317,389)(H,318,375)(H,338,339)(H,340,341)(H,342,343)(H,344,345)(H,391,392)(H,393,394)(H4,264,265,268)/t131-,132-,133-,134-,135+,149-,150-,151-,152-,153-,154-,155-,156-,157-,158-,159-,160-,161-,162-,163-,164-,165-,166-,167-,168-,169-,170-,171-,172-,173-,174-,175-,176-,177-,178-,179-,180-,181-,182-,183-,184-,185-,200-,201-,202-,203-,204-,205-,206-/m0/s1
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This Item | |||
|---|---|---|---|
| biological source bovine | biological source - | biological source bovine | biological source - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| product line BioXtra | product line - | product line - | product line - |
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | hybridoma: suitable | technique(s) liquid chromatography (LC): suitable |
| form lyophilized powder | form powder | form powder | form liquid |
| solubility acetic acid: 2 mg/mL (pH: 2-3), hydrochloric acid: 2 mg/mL (pH: 2-3) | solubility water: 2 mg/mL, clear, colorless (pH: 2) | solubility acidified water, pH 2.0: 2 mg/mL | solubility - |
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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how to soluble i1882? What percent dilution should I use to gial acetic acid?
1 answer-
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What can be used to make a solution of insulin?
1 answer-
Insulin is soluble in dilute acids and alkalis. The solubility of insulin is tested at 2 mg/mL in water (acidified to pH 2 with hydrochloric acid). One can also use 1 mL of water acidified to pH 2 with 10 μL of glacial acetic acid to dissolve 2 mg of insulin. A 10 mg/mL solution of bovine insulin in 25 mM HEPES at pH 8.2 is offered as Product No. I0516.
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How many units of insulin are present in one vial of Product I1882, Insulin from bovine pancreas?
1 answer-
Product No. I1882 has a potency specification of not less than 2520 USP units per vial. For a vial that contains (nominally) 100 mg, that works out to 25.2 USP units/mg. The potency in terms of units per vial can be found the on lot-specific Certificate of Analysis.
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How can I store the insulin solution? How long?
1 answer-
The USP recommends that solutions of insulin be stored at 2-8°C. It is not recommended to freeze solutions of insulin, as it can aggregate. Sterile solutions should be stable for at least 1 year at 2-8°C.
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What is the comparison between International Units (IU) and USP units for insulin?
1 answer-
The international unit (IU) of insulin is based on the International Standard Preparation of Bioassay (1958). The standard preparation is a mixture of 52% bovine and 48% porcine insulin, containing 24 units/mg. Reference: Martindale: The Extra Pharmacopoeia, 29th ed., p. 392. High purity bovine pancreatic insulin typically assays at approximately 27 USP units per mg. So the IU and USP unit are not identical, but they are approximately 1:1.
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What is the amino acid sequence for Product I1882, Insulin from bovine pancreas?
1 answer-
The sequence and structure of insulin can be found on the product information sheet (under Documents, above).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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