Specificity for the m-chain of human IgM is determined by Ouchterlony Double Diffusion (ODD). The antibody preparation is specific for human IgM when tested against purified human IgA, IgG, IgM, Bence Jones kappa, and Bence Jones lambda myeloma proteins. Identity and purity of the antibody is established by immunoelectrophoresis (IEP). Electrophoresis of the antibody preparation followed by diffusion versus antigoat IgG and anti-goat whole serum result in single arcs of precipitation. One milligram of affinity isolated antigen specific antibody will react with 0.5-5.0 mg of human IgM as determined by single radial immunodiffusion.
I0759
Anti-Human IgM (μ-chain specific) antibody produced in goat
affinity isolated antibody, lyophilized powder
Synonym(s):
Anti Human IgM Antibody - Anti-Human IgM (μ-chain specific) antibody produced in goat, Anti Human Igm, Anti Human Igm Antibody, Anti-Human Igm
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About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ODD, QPA
Citations:
28
Pricing and availability is not currently available.
biological source
goat
Quality Segment
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
lyophilized powder
technique(s)
Ouchterlony double diffusion: suitable, quantitative precipitin assay: suitable
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Human serum-derived IgM may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents.
Immunoglobulin M (IgM) antibodies appear early in the course of infections. IgM antibodies are responsible for agglutination of red blood cells in mis-matched blood transfusions. The level of IgM may vary with the status of disease or infection.
Immunoglobulin M (IgM) antibodies appear early in the course of infections. IgM antibodies are responsible for agglutination of red blood cells in mis-matched blood transfusions. The level of IgM may vary with the status of disease or infection.
IgM antibodies are present as pentamers in the serum and are produced in response to antigens
Anti-Human IgM (μ-chain specific) antibody is specific for human IgM with mu chain. The antibody preparation is specific for human IgM when tested against purified human IgA, IgG, IgM, Bence Jones κ and Bence Jones λ myeloma proteins.
Anti-Human IgM (μ-chain specific) antibody is specific for human IgM with mu chain. The antibody preparation is specific for human IgM when tested against purified human IgA, IgG, IgM, Bence Jones κ and Bence Jones λ myeloma proteins.
Immunogen
Purified human IgM as immunogen
Application
Anti-Human IgM (μ-chain specific) antibody is suitable for Ouchterlony double diffusion and quantitative precipitin assay. It was used in B-cell receptor cross-linking studies, AuBioDOT assays, ELISA, protein purification and gel blot analysis.
Goat polyclonal Anti-Human IgM (μ-chain specific) antibody may be used to identify and quantitate the level of human IgM in vitro and in human serum or other biological fluids by immunochemical or immunohistological techniques.
Biochem/physiol Actions
Goat polyclonal Anti-Human IgM (μ-chain specific) antibody reacts with the m-chain of human IgM. It is specific for human IgM when tested against purified human IgA, IgG, IgM, Bence Jones kappa and Bence Jones lambda myeloma proteins.
Physical form
Lyophilized from 0.01 M sodium phosphate, 0.015 M sodium chloride, pH 7.2
Preparation Note
Reconstitute with 0.135 M sodium chloride.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| technique(s) Ouchterlony double diffusion: suitable, quantitative precipitin assay: suitable | technique(s) Ouchterlony double diffusion: suitable, immunoelectrophoresis: suitable | technique(s) direct ELISA: 1:10,000 | technique(s) Ouchterlony double diffusion: suitable |
| antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form affinity isolated antibody |
| conjugate unconjugated | conjugate agarose conjugate | conjugate peroxidase conjugate | conjugate unconjugated |
| biological source goat | biological source goat | biological source goat | biological source goat |
| form lyophilized powder | form PBS suspension | form buffered aqueous solution | form lyophilized powder |
| clone polyclonal | clone polyclonal | clone polyclonal | clone polyclonal |
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Quellles sont la nature et la licalisation de l'epitope ciblé par cet AC sur la chaîne lourde humaine ?
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