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H7039

Sigma-Aldrich

3-Hydroxy-3-methylglutaryl-CoA reductase human

solution

Synonym(s):

HMGCoA Reductase, HMGR

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About This Item

Enzyme Commission number:
1.1.1.34 (BRENDA, IUBMB)
UNSPSC Code:
12352204
NACRES:
NA.54

Assay

≥90% (SDS-PAGE)

Quality Level

200

form

solution

specific activity

2-8 units/mg protein

mol wt

~76 kDa (for the fusion protein)

UniProt accession no.

P04035

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... HMGCR(3156)

Application

3-Hydroxy-3-methylglutaryl-CoA reductase human has been used:
  • in anti-HMGCR enzyme linked immunosorbent assay (ELISA)
  • as an antigen to measure anti-HMGCR antibodies by blot analysis
  • in HMG-CoA reductase activity assays
  • as an antigen to compare in-house antigen via sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE)/western blot

Biochem/physiol Actions

3-Hydroxy-3-methylglutaryl-CoA reductase/HMGCR serves as a rate limiting enzyme in mevalonate pathway. It also controls the oncogenic Hippo transducers TAZ/YAP. HMGCR also perform as a genetic modifier for Alzheimer′s disease (AD).
Hydroxy-3-methylglutaryl-CoA reductase catalyzes the committed step in cholesterol biosynthesis.

Physical properties

Consists of the HMG CoA reductase catalytic domain fused to GST protein.

Unit Definition

One unit will convert 1.0 μmole of NADPH to NADP+ per minute at 37 °C.

Physical form

Supplied as a solution containing 250 μg protein in 50 mM Tris pH 7.5, with 5 mM DTT, 1:200 Protease Inhibitor Cocktail (Catalog Number P8340) and 50% (w/v) glycerol.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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HMG-CoAR expression in male breast cancer: relationship with hormone receptors, Hippo transducers and survival outcomes
Di BA, et al.
Scientific reports, 6, 35121-35121 (2016)
Myopathy with anti-HMGCR antibodies Perimysium and myofiber pathology
Alshehri A, et al.
Neurology? neuroimmunology & neuroinflammation, 2(4), e124-e124 (2015)
Association of HMGCR polymorphism with late-onset Alzheimer's disease in Han Chinese
Chang XL, et al.
Oncotarget, 7(16), 22746-22751 (2016)
Marie Hudson et al.
Medicine, 95(44), e5280-e5280 (2016-11-20)
The objective of this study was to investigate the frequency of autoantibodies to hydroxymethylglutaryl coenzyme A reductase (HMGCR) in systemic sclerosis (SSc) and associations with inflammatory myositis and statin exposure.This was a cross-sectional, multicenter study of 306 subjects from the
OP0009 Distinctive Immunofluorescence Pattern in Statin-Associated Autoimmune Myopathy with Anti-HMGCR Autoantibodies
Alvarado-Cardenas M, et al.
Annals of the Rheumatic Diseases, 75, 56-56 (2016)
View All Related Papers

Articles

Cholesterol Biosynthesis

Cholesterol biosynthesis starts in the hepatic endoplasmic reticulum with acetyl-CoA, yielding 3-hydroxy-3-methylglutaryl-CoA via HMG-CoA synthase.

Cholesterol Biosynthesis

Cholesterol biosynthesis starts in the hepatic endoplasmic reticulum with acetyl-CoA, yielding 3-hydroxy-3-methylglutaryl-CoA via HMG-CoA synthase.

Cholesterol Biosynthesis

Cholesterol biosynthesis starts in the hepatic endoplasmic reticulum with acetyl-CoA, yielding 3-hydroxy-3-methylglutaryl-CoA via HMG-CoA synthase.

Cholesterol Biosynthesis

Cholesterol biosynthesis starts in the hepatic endoplasmic reticulum with acetyl-CoA, yielding 3-hydroxy-3-methylglutaryl-CoA via HMG-CoA synthase.

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