F7884

Anti-Dog IgG (whole molecule)–FITC antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Rabbit Anti-Dog IgG (whole molecule)–Fluorescein isothiocyanate
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00221535
• Conjugate: FITC conjugate
• Clone: polyclonal
• Application: IF (d)
• Citations: 11

Properties

• biological source: rabbit
• Quality Segment: 300
• conjugate: FITC conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• storage condition: protect from light
• technique(s): direct immunofluorescence: 1:32
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

IgG (Immunoglobulin G) antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Rabbit anti-dog IgG is isolated by affinity isolation and conjugated to Fluorescein Isothiocyanate (FITC).
The variable region of IgG antibody is specific to antigens and is highly conserved. IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c.

Immunogen

Purified dog IgG

Application

Anti-Dog IgG (whole molecule)-FITC antibody may be used for direct immunofluorescence using dog peripheral blood lymphocytes at a minimum working dilution of 1:32.

Biochem/physiol Actions

Anti-Dog IgG (whole molecule)-FITC antibody is specific for dog IgG subclasses.

IgG (Immunoglobulin G) antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. IgG antibody has its function similar to IgM antibody in complement system activation. IgG participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month.
For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)