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F6636

Nutrient Mixture F-12 Ham

Coon′s Modification, with ʟ-glutamine and zinc sulfate, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

Ham’s F-12

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217417
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Product Name

Nutrient Mixture F-12 Ham, Coon′s Modification, with L-glutamine and 0.863 mg/L zinc sulfate, without sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

components

phenol red: 0.00125 g/L
L-glutamine: 0.292 g/L
sodium pyruvate: 0.22 g/L
glucose: 1.802 g/L

shipped in

ambient

storage temp.

2-8°C

Application

Coon's modification of Ham's F-12 was developed for culturing hybrid cells that were produced by viral fusion. The modification consists of doubling the amino acids and pyruvate and including ascorbic acid. The salt concentrations have also been altered. The formula contains 0.863 mg/L zinc sulfate, which may render it unsuitable for culturing mouse L-cells.

Preparation Note

Formulated to contain 11.5 grams of powder per liter of medium.
Supplement with 2.68 g/L sodium bicarbonate.

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SAFC

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F6636

Nutrient Mixture F-12 Ham

Sigma-Aldrich

Sigma-Aldrich

N6760

Nutrient Mixture F-12 Ham

Sigma-Aldrich

Sigma-Aldrich

N8641

Nutrient Mixture F-12 Ham

Sigma-Aldrich

Sigma-Aldrich

N3520

Nutrient Mixture F-12 Ham

form

powder

form

powder

form

liquid

form

powder

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

phenol red: 0.00125 g/L
L-glutamine: 0.292 g/L
sodium pyruvate: 0.22 g/L
glucose: 1.802 g/L

components

HEPES: no
sodium pyruvate: 0.11 g/L
phenol red: yes
L-glutamine: yes
NaHCO3: no

components

L-glutamine: no
sodium pyruvate: 0.11 g/L
NaHCO3: yes
phenol red: yes
HEPES: yes

components

NaHCO3: no
sodium pyruvate: 0.22 g/L
HEPES: no
L-glutamine: yes
phenol red: yes

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

hybrid cell cultue, amino acid enriched

Low serum media

-

differentiated cell culture medium, primary human liver cell cultuer medium

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

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Questions

  1. What is the recommended pH of this media?

    1 answer

    1. The pH specification range for this product is 5.4 - 6.0, however this is not suitable as a final pH for the culture of cells. This formulation requires the addition of sodium bicarbonate as a buffering agent. The final pH will be adjusted using an acid or base to reach the desired final pH, typically 7.2 - 7.4. See the links below to review a sample Certificate of Analysis as well as the product datasheet which includes preparation instructions.

      Product datasheet
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/124/814/f6636pis.pdf

      Sample Certificate of Analysis
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/F6636-10X1LSLCP6322.pdf

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