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F1262

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment–FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

indirect immunofluorescence: 1:40

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Goat polyclonal anti-Rabbit IgG (whole molecule), F(ab′)2 fragment–FITC antibody binds all rabbit IgGs.
Immunoglobulins (Igs) are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs can be useful tools for the analysis of target proteins
Anti-Rabbit IgG (whole molecule), (F(ab′)2) fragment-FITC is specific for all rabbit immunoglobulins. The use of this product prevents background staining due to the presence of Fc receptors.
Rabbit IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rabbit serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.

Immunogen

Purified rabbit IgG

Application

Anti-Rabbit IgG (whole molecule), (F(ab′)2) fragment-FITC is suitable for use in flow cytometry.
Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment–FITC antibody produced in goat has also been used for fluorescence labeling and immunocytochemistry.
Goat polyclonal anti-Rabbit IgG (whole molecule), F(ab′)2 fragment–FITC antibody may be used to detect and quantitate the level of IgG in rabbut serum and biological fluids by fluorescent techniques. It may also be used as a secondary antibody in assays that use rabbit IgG as the primary antibody. F(ab′)2 fragment–FITC antibody is useful when trying to avoid background staining due to the presence of Fc receptors.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Synergistic induction of PI-PLCβ1 signaling by azacitidine and valproic acid in high-risk myelodysplastic syndromes
Follo MY, et al.
Leukemia, 25(2), 271-271 (2011)
Edith Terrenoire et al.
Genome biology, 11(11), R110-R110 (2010-11-17)
Immunolabeling of metaphase chromosome spreads can map components of the human epigenome at the single cell level. Previously, there has been no systematic attempt to explore the potential of this approach for epigenomic mapping and thereby to complement approaches based
Anne Rosbjerg et al.
Frontiers in immunology, 7, 473-473 (2016-11-20)
Aspergillus fumigatus infections are associated with a high mortality rate for immunocompromised patients. The complement system is considered to be important in protection against this fungus, yet the course of activation is unclear. The aim of this study was to
Petri Elo et al.
Journal of neuroinflammation, 15(1), 128-128 (2018-05-03)
Vascular adhesion protein-1 (VAP-1) is an inflammation-inducible endothelial cell molecule and primary amine oxidase that mediates leukocyte entry to sites of inflammation. However, there is limited knowledge of the inflammation-related expression of VAP-1 in the central nervous system (CNS). Therefore
Edith Terrenoire et al.
BMC genetics, 16, 44-44 (2015-05-01)
Using metaphase spreads from human lymphoblastoid cell lines, we previously showed how immunofluorescence microscopy could define the distribution of histone modifications across metaphase chromosomes. We showed that different histone modifications gave consistent and clearly defined immunofluorescent banding patterns. However, it

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