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Key Documents

D4788

Sigma-Aldrich

2′-Deoxyadenosine 5′-triphosphate sodium salt solution

100 mM, pH 7

Synonym(s):

dATP

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About This Item

EC Number:
UNSPSC Code:
41106305
eCl@ss:
32160414
PubChem Substance ID:
NACRES:
NA.52

Assay

≥99%

Quality Level

form

liquid

concentration

100 mM

color

colorless

pH

7

foreign activity

DNase, RNase, none detected

shipped in

dry ice

storage temp.

−20°C

SMILES string

[Na+].Nc1ncnc2n(cnc12)[C@H]3C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)([O-])=O)O3

InChI

1S/C10H16N5O12P3.Na/c11-9-8-10(13-3-12-9)15(4-14-8)7-1-5(16)6(25-7)2-24-29(20,21)27-30(22,23)26-28(17,18)19;/h3-7,16H,1-2H2,(H,20,21)(H,22,23)(H2,11,12,13)(H2,17,18,19);/q;+1/p-1/t5-,6+,7+;/m0./s1

InChI key

YJWCICGGRLOGEH-VWZUFWLJSA-M

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General description

2′-Deoxyadenosine 5′-triphosphate (dATP) is made up of a nucleobase attached to deoxyribose and a 5′-hydroxyl on the sugar bound to a chain of three phosphate residues. dATP is used by cells for synthesis of DNA by DNA polymerases.

Application

2′-Deoxyadenosine 5′-triphosphate sodium salt solution has been used in the detection of apoptotic cells by the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) technique. It has also been used to induce oligomerization of apoptotic protease activating factor-1 (Apaf-1) to form a large heptameric complex referred to as the apoptosome or Apaf-1 procaspase-9 apoptosome complex and to study its functions as a proteolytic-based molecular timer.
2′-Deoxyadenosine 5′-triphosphate sodium salt solution is used in DNA sysnthesis reactions such as PCR, DNA sequencing and molecular cloning techniques.

Components

T4 DNA Ligaes is supplied in a solution containing 20 mM Tris-HCl(ph 7.5), 50mM KCl, 1mM DTT, and 50% (v/v) glycerol

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ultrasound-guided intramural inoculation of orthotopic bladder cancer xenografts: a novel high-precision approach
Jager W, et al.
Testing, 8(3), e59536-e59536 (2013)
Srinivas Malladi et al.
The EMBO journal, 28(13), 1916-1925 (2009-06-06)
During stress-induced apoptosis, the initiator caspase-9 is activated by the Apaf-1 apoptosome and must remain bound to retain significant catalytic activity. Nevertheless, in apoptotic cells the vast majority of processed caspase-9 is paradoxically observed outside the complex. We show herein
Anders Hofer et al.
Critical reviews in biochemistry and molecular biology, 47(1), 50-63 (2011-11-05)
Ribonucleotide reductase (RNR) is the only source for de novo production of the four deoxyribonucleoside triphosphate (dNTP) building blocks needed for DNA synthesis and repair. It is crucial that these dNTP pools are carefully balanced, since mutation rates increase when
Kevin N Kirouac et al.
Nucleic acids research, 41(3), 2060-2071 (2012-12-27)
Nitrated polycyclic aromatic hydrocarbons are common environmental pollutants, of which many are mutagenic and carcinogenic. 1-Nitropyrene is the most abundant nitrated polycyclic aromatic hydrocarbon, which causes DNA damage and is carcinogenic in experimental animals. Error-prone translesion synthesis of 1-nitropyrene-derived DNA
Serdal Kirmizialtin et al.
Structure (London, England : 1993), 20(4), 618-627 (2012-04-10)
Nearly every enzyme undergoes a significant change in structure after binding it's substrate. Experimental and theoretical analyses of the role of changes in HIV reverse transcriptase structure in selecting a correct substrate are presented. Atomically detailed simulations using the Milestoning

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