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D0776

Sigma-Aldrich

2′-Deoxycytidine hydrochloride

BioReagent, suitable for cell culture

Synonym(s):

1-(2-Deoxy-β-D-ribofuranosyl)cytosine hydrochloride, Cytosine deoxyriboside hydrochloride

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About This Item

Empirical Formula (Hill Notation):
C9H13N3O4 · HCl
CAS Number:
Molecular Weight:
263.68
Beilstein:
3576124
EC Number:
MDL number:
UNSPSC Code:
12352207
PubChem Substance ID:
NACRES:
NA.75

biological source

synthetic

product line

BioReagent

Assay

≥99% (HPLC)

form

powder

technique(s)

cell culture | mammalian: suitable

solubility

water: 50 mg/mL, clear, colorless

SMILES string

Cl[H].NC1=NC(=O)N(C=C1)[C@H]2C[C@H](O)[C@@H](CO)O2

InChI

1S/C9H13N3O4.ClH/c10-7-1-2-12(9(15)11-7)8-3-5(14)6(4-13)16-8;/h1-2,5-6,8,13-14H,3-4H2,(H2,10,11,15);1H/t5-,6+,8+;/m0./s1

InChI key

LTKCXZGFJFAPLY-OERIEOFYSA-N

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Application

2′-Deoxycytidine hydrochloride has been used:
  • as a deoxyribonucleoside component in the basal medium to study its effects on Bacillus mojavensis′s anaerobic growth
  • in the infectivity assay of monocyte-derived macrophages by human immunodeficiency virus 1 (HIV-1)
  • in nucleoside supplementation for the synchronization of human retinal pigment epithelial-1 (RPE-1) cells in G1 phase

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Md Maminur Rahman et al.
The Journal of biological chemistry, 295(51), 17460-17475 (2021-01-18)
Homologous recombination (HR) repairs DNA double-strand breaks using intact homologous sequences as template DNA. Broken DNA and intact homologous sequences form joint molecules (JMs), including Holliday junctions (HJs), as HR intermediates. HJs are resolved to form crossover and noncrossover products.
Fuxia Li et al.
American journal of cancer research, 10(3), 908-924 (2020-04-09)
Epithelial ovarian cancer is characterized by universal TP53 mutations, which result in G1/S checkpoint deficiencies. Therefore, it is hypothesized that the abrogation of the G2/M checkpoint with Wee1 inhibitor might preferentially sensitize TP53-defective ovarian cancer cells. Given the extremely high
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Aiman A Haqqani et al.
Virology journal, 12, 184-184 (2015-11-13)
Template switching between two distinct HIV-1 RNA genomes during reverse transcription gives rise to recombinant viruses that greatly expand the genetic diversity of HIV-1 and have adverse implications for drug resistance, immune escape, and vaccine design. Virions with two distinct
Harry E Taylor et al.
Cell reports, 31(12), 107810-107810 (2020-06-25)
Cellular metabolism governs the susceptibility of CD4 T cells to HIV-1 infection. Multiple early post-fusion steps of HIV-1 replication are restricted in resting peripheral blood CD4 T cells; however, molecular mechanisms that underlie metabolic control of these steps remain undefined. Here, we

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