D0550

3,5-Dinitrosalicylic acid

used in colorimetric determination of reducing sugars

• Synonym(s): 3,5-Dinitro-2-hydroxybenzoic acid, DNS
• Linear Formula: (O₂N)₂C₆H₂-2-(OH)CO₂H
• CAS Number: 609-99-4
• Molecular Weight: 228.12
• UNSPSC Code: 12171500
• NACRES: NA.47
• PubChem Substance ID: 24893243
• EC Number: 210-204-3
• Beilstein/REAXYS Number: 2220661
• MDL number: MFCD00007104
• Assay: ≥98% (HPLC and titration)
• Form: powder

Properties

• Quality Segment: 200
• assay: ≥98% (HPLC and titration)
• form: powder
• mp: 168-172 °C (lit.)
• solubility: H₂O: 50 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: OC(=O)c1cc(cc(c1O)[N+]([O-])=O)[N+]([O-])=O
• InChI: 1S/C7H4N2O7/c10-6-4(7(11)12)1-3(8(13)14)2-5(6)9(15)16/h1-2,10H,(H,11,12)
• InChI key: LWFUFLREGJMOIZ-UHFFFAOYSA-N

Description

Application

3,5-Dinitrosalicylic acid (DNS) has been used to stop the reaction in in vitro α-amylase inhibition study.[1] It has also been used for the quantitation of enzymatically released reducing sugars.

Biochem/physiol Actions

3,5-Dinitrosalicylic acid (DNS) is used in colorimetric determination of reducing sugars[2] and to analyze glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. The dinitrosalicylic acid method has been compared to the Nelson-Somogi colorimetric method.

Safety Information

• pictograms: GHS05,GHS07
• signalword: Danger
• hcodes: H302,H318
• pcodes: P264 - P270 - P280 - P301 + P312 - P305 + P351 + P338 - P501
• Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1
• Storage Class: 4.1A - Other explosive hazardous materials
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves