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Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

Name: Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham
Brand: SIGMA

With L-glutamine and trace elements, without HEPES and sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

DME/F-12, 1:1 mixture

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.71

MDL number:

MFCD00217417

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Properties

Quality Segment

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO₃: no
sodium pyruvate: 0.11 g/L
HEPES: no
phenol red: yes
glucose: 3.15 g/L
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

Description

General description

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is a 1:1 mixture.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used:

to culture cerebral cortices from mice embryos
as a component of the human corneal epithelium (HCE) media for culturing human limbal explants
to culture human corneal epithelial cell lines[1]

Preparation Note

Formulated to contain 12.0 grams of powder per liter of medium.

Supplement with 1.2 g/L sodium bicarbonate

Other Notes

Trace elements include ammonium molybdate, ammonium metavanadate, stannous chloride, sodium selenite, magnesium sulfate, nickel chloride, and sodium metasilicate

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This Item	D8900	D2906	FG4815
Sigma-Aldrich D0547 Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham	Sigma-Aldrich D8900 Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham	Sigma-Aldrich D2906 Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham	Sigma-Aldrich FG4815 Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham
form powder	form powder	form powder	form liquid
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
components NaHCO₃: no sodium pyruvate: 0.11 g/L HEPES: no phenol red: yes glucose: 3.15 g/L L-glutamine: yes	components sodium pyruvate: 0.055 g/L HEPES: 15 mM NaHCO₃: no L-glutamine: yes glucose: 3.15 g/L phenol red: yes	components NaHCO₃: no L-glutamine: yes phenol red: no sodium pyruvate: 0.055 g/L glucose: 3.15 g/L HEPES: 15 mM	components phenol red: yes stable glutamine: yes glucose: high HEPES: no NaHCO₃: yes sodium pyruvate: yes
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
Quality Level 500	Quality Level 500	Quality Level 500	Quality Level 400

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