Our lab has not tested pET-22b with CMC0018 C41(DE3) pLysS Chemically Competent Cells. However, according to the following publication, pET-22b seems to work well with C41(DE3) cells: Protein Expr Purif. 2007 Sep;55(1):189-97. doi: 10.1016/j.pep.2007.04.017. Epub 2007 Apr 29. The publication is titled "Cloning and structural analysis of Mycobacterium leprae serine hydroxymethyltransferase" with the PMID: 17540580.
CMC0018
OverExpress™ C41(DE3) pLysS Chemically Competent Cells
Escherichia coli, rod shaped
Synonym(s):
Chemically competent cells
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About This Item
UNSPSC Code:
41106202
NACRES:
NA.85
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Product Name
OverExpress™ C41(DE3) pLysS Chemically Competent Cells, for the highest protein expression
biological source
Escherichia coli
grade
for molecular biology
growth mode
adherent or suspension
morphology
rod shaped
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: chemically competent
transformation efficiency: ≥1 x 106 cfu/μg
shipped in
dry ice
storage temp.
−70°C
Related Categories
General description
OverExpress™ Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications. The OverExpress™ strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3). OverExpress C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. Cells containing pLysS produce a small amount of T7 lysozyme. These strains are used to suppress basal expression of T7 RNA polymerase prior to induction, thus stabilizing recombinants encoding particularly toxic proteins.
Genotype
F – ompT hsdSB (rB- mB-) gal dcm (DE3) pLysS (CmR)
Genotype
F – ompT hsdSB (rB- mB-) gal dcm (DE3) pLysS (CmR)
Features and Benefits
- Express genes cloned into any T7 vector with these BL21(DE3) derivatives
- Effective in expressing toxic & membrane proteins
- Cited in over 350 research articles
Components
- OverExpress C41(DE3) pLysS chemically competent cells
- pUC 19 transformation control DNA
- recovery medium for expression
Legal Information
OverExpress is a trademark of Imaxio, S.A.
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Storage Class Code
10 - Combustible liquids
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Protocols
Protocol for OverExpress™ Chemically Competent Cells. Product Numbers: CMC0017, CMC0018, CMC0019, CMC0020, CMC0023, CMC0024
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Can C43 competent cells produce periplasmic proteins using pET22b? .
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