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BRIN-BG5

10033001, rat pancreas (islets), Epitheloid

Synonym(s):

BRIN

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About This Item

UNSPSC Code:
41106514

product name

BRIN-BG5, 10033001

biological source

rat pancreas (islets)

growth mode

Adherent

karyotype

Modal chromosome number 66 up to passage 50

morphology

Epitheloid

products

BRIN-BG5 is a stable, glucose-responsive insulin secreting beta cell line expressing insulin, glucokinase and IAPP as detected by immunocytochemistry. BRIN-BG5 cells express the GLUT2 glucose transporter.

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

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Cell Line Origin

A rat pancreatic Beta cell: RINm5F hybrid cell line. Insulin-secreting cell line

Cell Line Description

BRIN-BG5 is a hybrid cell line formed by the electrofusion of a primary culture of NEDH rat pancreatic islets with RINm5F (a cell line derived from a NEDH rat insuliinoma). BRIN-BG5 has been shown to be tumourigenic when transplanted into a SCID mouse host. BRIN-BG5 has been mycoplasma eradicated at ECACC and the stocks available for supply have undergone a further 10 passages without detection of mycoplasma.

Culture Medium

RPMI 1640 (R0883) + 2 mM L-Glutamine (G7513) + FBS / FCS (F2442)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:8 to 1:10, i.e. seeding at 2-4 x 104 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Population doubling approximately28hrs. At confluence 105 cells/cm2 can be expected.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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