Cholera Toxin A Subunit from Vibrio cholerae

Name: Cholera Toxin A Subunit from <I>Vibrio cholerae</I>
Brand: SIGMA

lyophilized powder

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About This Item

UNSPSC Code:

12352200

NACRES:

NA.77

MDL number:

MFCD00130777

Form:

lyophilized powder

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Properties

form

lyophilized powder

Quality Segment

200

composition

Protein, ~5% Lowry

availability

not available in Japan

impurities

≤0.5% Cholera toxin B subunit (SDS-PAGE)

storage temp.

2-8°C

Description

General description

Cholera Toxin (CT) is secreted by Vibrio cholerae. CT upregulates cell surface molecules, such as co-stimulatory molecules and chemokine receptors in antigen presenting cells(APCs), murine and human dendritic cells (DCs). It enhances the secretion of interleukin 1 (IL-1) from macrophages and stimulates their APC function. CT also functions as an immunomodulator.

Application

Cholera Toxin A Subunit from Vibrio cholerae has been used as a specific probe for trimeric G proteins in membrane motility assays.

Biochem/physiol Actions

Catalyzes ADP-ribosylation of the α-subunit of G proteins, reducing GTPase activity and activating the α-subunit; also catalyzes ADP-ribosylation of cell membrane adenylyl cyclase

Physical form

Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide and sodium EDTA

Preparation Note

When reconstituted with 1 mL of water, the solution will contain 0.05 M Tris buffer salts, pH 7.5, 0.2 M NaCl, 0.003 M NaN₃, and 0.001 M sodium EDTA. Store reconstituted solutions in the refrigerator.