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C5041

Sigma-Aldrich

6-Carboxyfluorescein diacetate

~95% purity (HPLC), powder

Synonym(s):

6-CFDA, 6-Carboxy-di-O-acetylfluorescein

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About This Item

Empirical Formula (Hill Notation):
C25H16O9
CAS Number:
3348-03-6
Molecular Weight:
460.39
Beilstein:
67043
MDL number:
MFCD00036871
UNSPSC Code:
12171500
PubChem Substance ID:
24892748
NACRES:
NA.47

product name

6-Carboxyfluorescein diacetate, ≥95% (HPLC)

Quality Level

100
200

Assay

≥95% (HPLC)

form

powder

color

white to yellow

solubility

chloroform/ethanol (1:1): 9.80-10.20 mg/mL, clear, colorless to light yellow

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccc(cc45)C(O)=O)c1

InChI

1S/C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25/h3-11H,1-2H3,(H,28,29)

InChI key

QMOGCCYGOPYYNT-UHFFFAOYSA-N

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Application

6-Carboxyfluorescein diacetate has been used to differentiate viable cells from apoptotic cells. Live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin.
Fluorescent wavelengths λex 492nm; λemm 517nm in 0.1M Tris, pH 8.0.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sung-Uk Lee et al.
PloS one, 14(3), e0214253-e0214253 (2019-03-27)
N-acetylglucosamine (GlcNAc) branching of Asn (N)-linked glycans inhibits pro-inflammatory T cell responses and models of autoimmune diseases such as Multiple Sclerosis (MS). Metabolism controls N-glycan branching in T cells by regulating de novo hexosamine pathway biosynthesis of UDP-GlcNAc, the donor
Yeli Gong et al.
PloS one, 7(10), e47435-e47435 (2012-10-19)
The MOG35-55 peptide-induced experimental autoimmune encephalomyelitis (EAE) model in C57BL/6 mice is a useful animal model to explore therapeutic approaches to T cell-mediated autoimmune diseases because the dominant T-cell epitope(s) have been defined. It is rational that antigen-specific immunosuppression can
Yuan Liu et al.
Journal of biomedical optics, 12(1), 014014-014014 (2007-03-09)
Intravital imaging of hepatobiliary excretion is vital for elucidating liver metabolism. In this work, we describe a novel method to observe the intravital dynamics of the uptake, processing, and excretion of an organic anion, 6-carboxyfluorescein diacetate (6-CFDA) in the hepatobiliary
M Kawai et al.
Journal of applied microbiology, 86(3), 496-504 (1999-04-10)
Physiologically active bacteria in purified water used in the manufacturing process of pharmaceutical products were enumerated in situ. Bacteria with growth potential were enumerated using the micro-colony technique and direct viable counting (DVC), followed by 24 h of incubation in
Evaluation of sperm quality snakes Erythrolamprus poecilogyrus sublineatus (Cope, 1860) (Serpentes, Dipsadidae).
Silva AC, et al.
Brazilian journal of biology = Revista brasleira de biologia, doi: 10-doi: 10 (2017)
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