B0390

Bilirubin Oxidase from Myrothecium verrucaria

lyophilized powder, 15-65 units/mg protein

• Synonym(s): MvBOx, Bilirubin:oxygen oxidoreductase
• CAS Number: 80619-01-8
• Enzyme Commission number: 1.3.3.5 (BRENDA, IUBMB)
• MDL number: MFCD00130650
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: fungus (Myrothecium verrucaria)
• form: lyophilized powder
• specific activity: 15-65 units/mg protein
• composition: Protein, ≥15% biuret
• storage condition: (Keep container tightly closed in a dry and well-ventilated place.)
• greener alternative product characteristics: Waste Prevention; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• technique(s): toxicology assay: suitable
• greener alternative category: , Enabling
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biofuel cell research. For more information see the article in biofiles.

Bilirubin oxidase (BOD) belongs to the subclass of the multicopper oxidase family. It is usually found in fungi.Bilirubin Oxidase is composed of three cupredoxin-like domains that result in two active sites formed by four copper ions. Research area: Apoptosis

Application

Bilirubin Oxidase from Myrothecium verrucaria has been used to eliminate the interference of bilirubin. It has also been used to chemically modify with fluorescein derivative (FS) and combined with multivariate calibration to analyse direct bilirubin (dBR) and total bilirubin (tBR).

Bilirubin oxidase is used to degrade bilirubin. Bilirubin oxidase, from Myrothecium verrucaria, may be used to determine free hemoglobin in icteric specimens . It also has potential application in dye effluent decolorization and is a potential treatment for neonatal jaundice.

Biochem/physiol Actions

Bilirubin oxidase (BOD) is a multicopper oxidase that catalyzes the oxidation of bilirubin to biliverdin. Oxygen is the electron acceptor and is reduced to water. The enzyme is used for diagnostic analysis of bilirubin in serum during medical examinations of the serum. It has also been used as an enzymatic catalyst for the cathode of biofuel cells that work under neutral conditions. It is a monomeric protein with a molecular mass of 60 kDa and high reactivity at neutral pH. BOD participates in porphyrin and chlorophyll metabolism.

Bilirubin oxidase (BOD) is capable of oxidizing laccase substrates.

Unit Definition

One unit will oxidize 1.0 μmole of bilirubin per min at pH 8.4 at 37 °C.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves