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A8781

β-Amylase from sweet potato

greener alternative

Synonym(s):

1,4-α-D-Glucan maltohydrolase

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About This Item

CAS Number:
9000-91-3
UNSPSC Code:
41106514
NACRES:
NA.56
EC Number:
232-566-1
MDL number:
MFCD00081391
EC Number:
3.2.1.2 (BRENDA, IUBMB)
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form

powder

Quality Level

100

mol wt

~200,000

packaging

vial of 15 mg

greener alternative product score

old score: 57
new score: 1
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greener alternative product characteristics

Waste Prevention
Safer Solvents and Auxiliaries
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

greener alternative category

Re-engineered,

storage temp.

−20°C

General description

β-Amylase from sweet potato is a tetrameric enzyme with each monomer comprising (α/β) core domain and a C-terminal loop region.[1] It belongs to the glycoside hydrolase family GH14.[2]
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Application

β-Amylase from sweet potato has been used:
  • as a molecular weight marker in blue native polyacrylamide gel electrophoresis (PAGE)[3]
  • as a standard for calibration curve preparation in size exclusion chromatography
  • as a model protein for small-angle X-ray analysis (SAXs)[4]

Biochem/physiol Actions

β-Amylase acts on the polysaccharide α(1,4) glycosidic linkages and releases maltose present in the non-reducing ends.[2] Due to its stability, β-Amylase from sweet potato is used in the immobilized enzyme preparation in combination with agarose beads.

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This Item
A7005A9857A6237
β-Amylase from sweet potato

Sigma-Aldrich

A8781

β-Amylase from sweet potato

β-Amylase from sweet potato Type I-B, ammonium sulfate suspension, ≥750 units/mg protein (E1%/280)

Sigma-Aldrich

A7005

β-Amylase from sweet potato

α-Amylase from Aspergillus oryzae ≥150 units/mg protein (biuret)

Sigma-Aldrich

A9857

α-Amylase from Aspergillus oryzae

Apyrase from potato recombinant, expressed in Pichia pastoris, ATPase ≥1000 units/mg protein, lyophilized powder

Sigma-Aldrich

A6237

Apyrase from potato

form

powder

form

ammonium sulfate suspension

form

powder

form

lyophilized powder

mol wt

~200,000

mol wt

-

mol wt

-

mol wt

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

greener alternative product characteristics

Waste Prevention
Safer Solvents and Auxiliaries
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Waste Prevention
Safer Solvents and Auxiliaries
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

-

packaging

vial of 15 mg

packaging

-

packaging

-

packaging

-

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000290697
0000290697
SLCQ9616
SLCQ9616
SLCP3236

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Khalilah G Reddie et al.
Molecular bioSystems, 4(6), 521-531 (2008-05-22)
Oxidation of the thiol functional group in cysteine (Cys-SH) to sulfenic (Cys-SOH), sulfinic (Cys-SO2H) and sulfonic acids (Cys-SO3H) is emerging as an important post-translational modification that can activate or deactivate the function of many proteins. Changes in thiol oxidation state
Luca Costa et al.
BMC structural biology, 16(1), 18-18 (2016-10-30)
Synchrotron radiation facilities are pillars of modern structural biology. Small-Angle X-ray scattering performed at synchrotron sources is often used to characterize the shape of biological macromolecules. A major challenge with high-energy X-ray beam on such macromolecules is the perturbation of
Determination of hydrodynamic radius of proteins by size exclusion chromatography
LaVV, et al.
Bio-protocol, 7(8), 14-Jan-14-Jan (2017)
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Global Trade Item Number

SKUGTIN
A8781-5X1VL04061833293225
A8781-1VL04061838212160

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