A6680

N-Acetylneuraminic Acid Aldolase from microorganisms

lyophilized powder, ≥20 units/mg protein (biuret)

• Synonym(s): N-Acetylneuraminate Pyruvate Lyase, N-Acetylneuraminic Acid Lyase, NANA Aldolase, Sialic Aldolase
• CAS Number: 9027-60-5
• NACRES: NA.32
• UNSPSC Code: 12352204
• Beilstein/REAXYS Number: 2697172
• MDL number: MFCD00131656
• EC Number: 4.1.3.3 (BRENDA, IUBMB)
• Specific activity: ≥20 units/mg protein (biuret)

Properties

• form: lyophilized powder
• Quality Segment: 200
• specific activity: ≥20 units/mg protein (biuret)
• mol wt: ~98 kDa
• storage temp.: −20°C

Description

General description

Isoelectric point: 4.6 ± 0.1
Michaelis constant: 2.5 x 10‾³M (N-Acetylneuraminic acid)
Structure: 3 subunits (approx. 35,000) per mol of enzyme
Inhibitors: p-Chloromercuribenzoate, sodium dodecyl sulfact, Hg⁺⁺, Ag⁺
Optimum pH: 7.5– 8.0
Optimum temp: 70°C
pH Stability: pH 6.0–9.0 (10°C, 25hr)
Thermal stability: Below 65°C (pH 7.5, 30 min)

Application

This enzyme is useful for enzymatic determination of N-acetylneuraminic acid and sialic acid when
coupled with the related enzymes in clinical analysis.
For industrial use, this enzyme is useful for enzymatic synthesis of sialic acid.

Used in the Sialic Acid Quantification Kit, SIALIC-Q

Physical form

Lyophilized powder containing mannitol and EDTA

Other Notes

One unit will release 1.0 μmole of pyruvate from NANA per min at pH 7.7 at 37 °C.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)