In catalog number FLAAS, the specification for our vial Content is 0.90 - 1.10 mg ATP/vial by Enzymatic Assay. Please refer to the specific lot certificate of analysis for an individual quantity.
A2383
Adenosine 5′-triphosphate disodium salt hydrate
≥99%, microbial, powder
Adenosine 5′-triphosphate disodium salt hydrate
Synonym(s):
ATP disodium salt
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About This Item
Empirical Formula (Hill Notation):
C10H14N5Na2O13P3 · xH2O
CAS Number:
Molecular Weight:
551.14 (anhydrous basis)
UNSPSC Code:
41106305
eCl@ss:
32160414
PubChem Substance ID:
NACRES:
NA.51
EC Number:
213-579-1
MDL number:
Assay:
≥99%
Biological source:
microbial
Form:
powder
Solubility:
H2O: 50 mg/mL
Storage temp.:
−20°C
Pricing and availability is not currently available.
Product Name
Adenosine 5′-triphosphate disodium salt hydrate, Grade I, ≥99%, from microbial
biological source
microbial
Quality Level
type
Grade I
assay
≥99%
form
powder
solubility
H2O: 50 mg/mL
storage temp.
−20°C
SMILES string
O.[Na+].[Na+].Nc1ncnc2n(cnc12)[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O
InChI
1S/C10H16N5O13P3.2Na.H2O/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(26-10)1-25-30(21,22)28-31(23,24)27-29(18,19)20;;;/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H,23,24)(H2,11,12,13)(H2,18,19,20);;;1H2/q;2*+1;/p-2/t4-,6-,7-,10-;;;/m1.../s1
InChI key
NTBQNWBHIXNPRU-MSQVLRTGSA-L
Application
Adenosine 5′-triphosphate disodium salt hydrate has been used:
- to prepare adenosine triphosphate (ATP) standard solutions to determine ATP levels in various bacterial cultures
- bone marrow-derived macrophages (BMDM) treatment[1]
- as a preparation free of contaminating guanine nucleotides to study the binding effects of coatomer β subunit (β-COP)[2]
Biochem/physiol Actions
Adenosine 5′-triphosphate (ATP) is a central component of energy storage and metabolism in vivo.[3] ATP is used in many cellular processes, respiration, biosynthetic reactions, active transport, and motility.[4] ATP is a substrate of many kinases involved in cell signaling and of adenylate cyclase that produce the secondary messenger cyclic adenosine monophosphate (cAMP). ATP provides the metabolic energy to drive metabolic pumps.[4] It serves as a coenzyme in a wide array of enzymatic reactions.
P2 purinergic agonist; increases activity of Ca2+-activated K+ channels; substrate for ATP-dependent enzyme systems
P2 purinocepter agonist.
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This Item | |||
|---|---|---|---|
| biological source microbial | biological source microbial | biological source microbial | biological source microbial |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| assay ≥99% | assay ≥97% (HPLC) | assay ≥99% (HPLC) | assay ≥99% (HPLC) |
| form powder | form crystalline | form powder | form powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| solubility H2O: 50 mg/mL | solubility H2O: 50 mg/mL | solubility H2O: 50 mg/mL | solubility H2O: 50 mg/mL, clear, colorless |
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type N95 (US)
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Related Content
J G Donaldson et al.
Proceedings of the National Academy of Sciences of the United States of America, 89(14), 6408-6412 (1992-07-15)
The coatomer is a cytosolic protein complex that reversibly associates with Golgi membranes and is implicated in modulating Golgi membrane transport. The association of beta-COP, a component of coatomer, with Golgi membranes is enhanced by guanosine 5'-[gamma-thio]triphosphate (GTP[gamma S]), a
Release of extracellular ATP by bacteria during growth
Mempin R, et al.
BMC Microbiology, 13(1), 301-301 (2013)
Rui Nian et al.
Biotechnology progress, 25(4), 1078-1085 (2009-06-25)
The use of polyethylene glycol (PEG) as a refolding additive to a refolding cocktail comprising the molecular bichaperone ClpB and DnaKJE significantly enhances chaperone-mediated refolding of heat-denatured malate dehydrogenase (MDH). The critical factor to affect the refolding yield is the
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A2383-25G | 04061833350065 |
| A2383-10G | 04061835553228 |
| A2383-100MG | 04061823206167 |
| A2383-5G | 04061835553235 |
| A2383-1G | 04061835515271 |
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I would like to know how many g of ATP disodium salt hydrate contains in FLAAS -1vol vial? Thanks
1 answer-
Helpful?
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What volume of water should 1mg ATP be reconstituted in & what would be its corresponding concentration?
1 answer-
This product is soluble in water up to 50 mg/mL. The appropriate stock concentration for assays can be determined by the end user. The molecular weight is 551.14 (anhydrous basis), and the water content can be found on the lot-specific certificate of analysis. Using the molecular weight, the water content, and the chosen concentration for the ATP solution, the molarity can be calculated.
Helpful?
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Can I dissolve this ATP in water and store it?
1 answer-
This item can be solubilized in water at 50 mg/mL yielding a clear, colorless solution. Aqueous solutions of ATP are stable for months at -15C and or one week when stored at -0C as mentioned in the Product Information Sheet.
For more information, please navigate to the ‘DOCUMENTATION’ section to access the Product Information Sheet under 'More Documents':
https://www.sigmaaldrich.com/product/sigma/a2383#product-documentationHelpful?
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