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SSC Buffer

Name: SSC Buffer
Brand: SIGMA

for Northern and Southern blotting, powder blend

Synonym(s):

Saline sodium citrate buffer dry blend

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About This Item

NACRES:

NA.25

UNSPSC Code:

12161700

Pricing and availability is not currently available.

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Properties

grade

Molecular Biology

Quality Segment

100

product line

BioReagent

form

powder blend

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

solubility

H₂O: 0.263 g/mL, clear, colorless

storage temp.

room temp

Description

General description

SSC Buffer powder is a standard reagent in Southern and Northern blotting procedures. This powder can be used to make SSC working solutions with concentrations ranging from 0.5x to 20x.

Application

Suitable for:

Preparing nucleic acids for hybridization
Hybridization buffer for Northern and Southern blots
Washing buffer for Northern and Southern blots

Preparation Note

Convenient pack sizes
Multi-purpose use in hybridizations
Easy-to-use powder makes fast, inexpensive solutions

Dissolve 263.5 g in a final volume of 1L molecular biology grade water. The resulting 20x concentrate is approximately pH 7, and can be further diluted with water, as needed.

Other Notes

SSC Buffer powder contains 0.3 M sodium citrate in 3M NaCl.

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This Item	S0902	PPB020	11666681001
Sigma-Aldrich 85639 SSC Buffer	Sigma-Aldrich S0902 SSC Buffer	Sigma-Aldrich PPB020 SSC Buffer	Roche 11666681001 Buffers in a Box, Premixed SSC Buffer, 20x
description for Northern and Southern blotting, powder blend	description for Northern and Southern blotting, powder blend	description pH 7.0, pHast Pack^™, powder	description pH 7.0, pkg of 4 L, solution
grade Molecular Biology	grade -	grade -	grade -
product line BioReagent	product line -	product line pHast Pack^™	product line -
Quality Level 100	Quality Level 200	Quality Level 100	Quality Level -
form powder blend	form powder blend	form powder	form solution
storage temp. room temp	storage temp. room temp	storage temp. -	storage temp. 20-25°C
solubility H₂O: 0.263 g/mL, clear, colorless	solubility water: 263 mg/mL, clear, colorless	solubility -	solubility -

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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