76258
Paraplast Plus®
suitable for electron microscopy, pellets (fast-melting)
Synonym(s):
Paraplast Plus® embedding media, Paraffin – polyisobutylene mixture
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About This Item
form
pellets (fast-melting)
Quality Level
technique(s)
electron microscopy: suitable
thickness
≤1 μm , short ribbons, ≤2 μm , sectioning: continuous ribbons
mp
56-57 °C
application(s)
hematology
histology
storage temp.
2-8°C
General description
Paraplast Plus® is a refined mixture of highly purified paraffin containing complex elastomer of regulated molecular weights plus a small percentage of dimethyl sulfoxide (DMSO) for fast tissue penetration. It produces minimal tissue compression and wrinkle-free sections and cuts to 4 μm thickness with excellent ribbon continuity.
Paraplast® tissue embedding media are made from highly purified wax combined with plastic polymers for tensile strength and elasticity. Paraplast Plus® provides rapid infiltration during processing as well as great compression resistance during sectioning for preserved morphology and thin, distortion-free sections.
Our range of Paraplast products improve the elasticity of brittle, difficult-to-cut tissues making it easier to produce excellent, wrinkle-free sections.
Paraplast® tissue embedding media are made from highly purified wax combined with plastic polymers for tensile strength and elasticity. Paraplast Plus® provides rapid infiltration during processing as well as great compression resistance during sectioning for preserved morphology and thin, distortion-free sections.
Our range of Paraplast products improve the elasticity of brittle, difficult-to-cut tissues making it easier to produce excellent, wrinkle-free sections.
Application
Paraffin polyisobutylene mixture is a tissue embedding medium composed of highly purified paraffin and polyisobutylene and a low level of dimethyl sulfoxide (DMSO) to enhance the rate of tissue penetration. Paraffin-polyisobutylene (76258) supports thin wrinkle-free sectioning down to 4 microM with more flexible and continuous ribbons.
Recommended for large specimens and tissue that commonly presents infiltration problems.
Features and Benefits
- Produces minimal tissue compression and wrinkle-free sections, and cuts to 4 μm thickness with excellent ribbon continuity.
- 0.8% DMSO enhances ease of infiltration and sectioning for tissues difficult to process with regular Paraplast.
- Higher melt viscosity and lower permeability to water vapor creates better adhesion and a more pliable, flexible film.
- Optimal infiltration and embedding for high-quality sections.
- Great compression resistance and ribbon continuity.
- Remarkable morphology preservation and distortion-free sections.
Legal Information
Paraplast is a registered trademark of Leica Biosystems
Paraplast Plus is a registered trademark of Leica Biosystems
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Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Ewa Urbańczyk-Wochniak et al.
Cellular & molecular biology letters, 7(1), 7-18 (2002-04-11)
This study describes an effective method of in situ RT-PCR (RT-ISPCR) that was developed to localize gene expression in plant tissues. This RT-PCR technique was performed on sectioned tissues of female buds of the cucumber GY3 inbred line. The CUS1
[Paraplast-plus, a tissue embedding medium that enables sections to stick to the slides without adhesives (author's transl)].
D Arn et al.
Mikroskopie, 31(3-4), 99-106 (1975-06-01)
Jing Qi et al.
Plant physiology, 147(4), 1947-1959 (2008-06-20)
The size and shape of the plant leaf is an important agronomic trait. To understand the molecular mechanism governing plant leaf shape, we characterized a classic rice (Oryza sativa) dwarf mutant named narrow leaf1 (nal1), which exhibits a characteristic phenotype
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 05-23-0500-25MG | 04055977206999 |
| 05-23-0500-5MG | 04055977207002 |
| 05-23-0500-1MG | 04055977206982 |
| 90834-10MG | 04061833252697 |
| B3259-25MG | 04061833427514 |
| B3259-100MG | 04061833427484 |
| B3259-1MG | 04061833427507 |
| B3259-10MG | 04061833427491 |
| B3259-5MG | 04061833427521 |
| 76258-1KG | 04061826714133 |