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GS Supplement 50X

Name: GS Supplement 50X
Brand: SIGMA

50 ×, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

GSEM Supplement, Glutamine synthetase expression medium supplement

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About This Item

NACRES:

NA.75

UNSPSC Code:

12352207

Pricing and availability is not currently available.

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Properties

description

for research or for further manufacturing use

Quality Segment

500

sterility

sterile-filtered

form

liquid

concentration

50 ×

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−20°C

Description

General description

The GS System^™ uses the Glutamine synthetase (GS) gene as a selectable marker for the expression of recombinant proteins in mammalian cells, especially NS0 and CHO cells. The system has been shown to have significant advantages with respect to speed and efficiency of cell line development and has resulted in high productivity for antibodies and other recombinant proteins.

Application

The glutamine synthetase (GS) system is used to express recombinant proteins from mammalian cells. Glutamine-dependent cells transfected with a glutamine synthetase gene can be selectively grown in a suitable glutamine-deficient medium.

GS Supplement 50X has been used to as a supplement for culture media to grow GS-NS0 mouse myeloma cells.

Packaging

Contains (mg/L): L-alanine 450.0, L-asparagine · H₂O 4261.0, L-aspartic acid 650.0, L-glutamic acid 3750.0, L-proline 575.0, L-serine 500.0, adenosine 350.0, guanosine 350.0, cytidine 350.0, uridine 350.0, and thymidine 12.0

Preparation Note

One mL will supplement 50 mL of cell culture medium.

Store GS Supplement 50X protected from light at -10 to -40 °C.

Legal Information

GS System is a trademark of Lonza Group Ltd.

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This Item	H0137	H0262	SCM081
SAFC 58672C GS Supplement 50X	Sigma-Aldrich H0137 HT Media Supplement (50×) Hybri-Max^™	Sigma-Aldrich H0262 HAT Media Supplement (50×) Hybri-Max^™	Sigma-Aldrich SCM081 N21 Medium Supplement (50X)
description 50 ×, liquid, sterile-filtered, suitable for cell culture	description lyophilized powder, γ-irradiated, BioXtra, suitable for hybridoma	description γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma	description The N21 Medium Supplement is an optimized serum-free supplement developed for isolation & expansion of neurons from mouse & rat hippocampus.
form liquid	form lyophilized powder	form lyophilized powder	form liquid
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| hybridoma: suitable	technique(s) cell culture \| hybridoma: suitable	technique(s) cell culture \| stem cell: suitable
sterility sterile-filtered	sterility γ-irradiated	sterility γ-irradiated	sterility -
concentration 50 ×	concentration -	concentration -	concentration -
Quality Level 500	Quality Level 200	Quality Level 200	Quality Level 100
description for research or for further manufacturing use	description -	description -	description -

Storage Class

12 - Non Combustible Liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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