89640

Toluidine Blue

8.74% (ZN (THEORY)), for microscopy (Hist., Vit.)

• Synonym(s): Tolonium chloride; Basic Blue 17
• Empirical Formula (Hill Notation): C₁₅H₁₆ClN₃S · 0.5ZnCl₂
• CAS Number: 6586-04-5
• Molecular Weight: 373.97
• Colour Index Number: 52040
• Beilstein: 4068052
• MDL number: MFCD03548293
• UNSPSC Code: 12171500
• PubChem Substance ID: 329769757
• NACRES: MA.02

Properties

• grade: for microscopy (Hist., Vit.)
• Quality Level: 200
• Assay: 8.74% (ZN (THEORY))
• form: powder or crystals
• color: green to very dark green
• solubility: methanol: 0.01 g/10 mL, blue to very deep blue
• εmax: ≥800 at 621-636 nm in water
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Cl-].[Cl-].Cl[Zn]Cl.Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C.Cc4cc5N=C6C=CC(\C=C6Sc5cc4N)=[N+](\C)C
• InChI: 1S/2C15H15N3S.4ClH.Zn/c2*1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;;;;;/h2*4-8,16H,1-3H3;4*1H;/q;;;;;;+2/p-2
• InChI key: DAQXTSXITPUGRG-UHFFFAOYSA-L

Description

General description

Toluidine blue (C.l. 52040) is a diaminothiazine dye.It is also known as TBO, tolonium chloride, and toluidine blue O.

Application

Toluidine Blue is suitable for use in the following applications:

1. to demonstrate amyloid; DNA in plant material, mast cells, Nissl substance, nuclei, and sulfatides
2. in metachromatic staining of sulfated mucins and related materials
3. as staining agent to identify the development and accumulation of the cartilage matrix, proteoglycans for isolation and culture of mesenchymal stem cells from mouse compact bone.
4. staining of mucins in water-miscible resins and of nucleic acids in epoxy resins(1)

Biochem/physiol Actions

Toluidine Blue is a basic thiazine metachromatic dye, which is used for selectively staining acidic tissue components such as carboxylates, sulfates, and phosphate radicals.It is found to have an affinity for nucleic acids and therefore binds to the nuclear material of tissues with a high DNA and RNA content.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves