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Tryptic Soy Broth - Dehydrated Culture Media

NutriSelect^® Plus, Broth No. 2, powder, suitable for microbiology

• Synonym(s): CASO Broth No. 2, Casein Soya Broth No. 2, Soybean Casein digest Broth No. 2, TSB No. 2, Tryptone Soya Broth No. 2, CASO Broth, Casein Soya Broth, Soybean Casein digest Broth, TSB, Tryptone Soya Broth
• UNSPSC Code: 41171607
• NACRES: NA.85
• MDL number: MFCD00132536
• Manufacturer/tradename: NutriSelect^® Plus
• Agency: according to ISO 10273:2017, according to ISO 21871:2006
• Form: powder
• Suitability: nonselective for Bacillus spp., nonselective for Bacteroides spp., nonselective for Campylobacter spp., nonselective for Candida spp., nonselective for Escherichia coli, nonselective for Neisseria spp., nonselective for Staphylococcus spp., nonselective for Streptococcus spp., nonselective for bacteria (General Media), nonselective for coliforms
• Application(s): bioburden testing; cleaning products; clinical testing; cosmetics; environmental; food and beverages; personal care; pharmaceutical; sample preparation; veterinary; water monitoring, microbiology

Properties

• Quality Segment: 200
• agency: according to ISO 10273:2017, according to ISO 21871:2006
• sterility: non-sterile
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: casein peptone, 17 g/L , dipotassium hydrogen phosphate, 2.5 g/L , glucose, 2.5 g/L , sodium chloride, 5 g/L , soya peptone, 3 g/L
• packaging: pack of 2.5 kg, pack of 500 g
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• color: beige
• final pH: 7.3±0.2 (25 °C)
• application(s): bioburden testing; cleaning products; clinical testing; cosmetics; environmental; food and beverages; personal care; pharmaceutical; sample preparation; veterinary; water monitoring, microbiology
• suitability: nonselective for Bacillus spp., nonselective for Bacteroides spp., nonselective for Campylobacter spp., nonselective for Candida spp., nonselective for Escherichia coli, nonselective for Neisseria spp., nonselective for Staphylococcus spp., nonselective for Streptococcus spp., nonselective for bacteria (General Media), nonselective for coliforms

Description

General description

Tryptic Soy Broth (TSB) is a general-purpose culture medium designed for the cultivation and enrichment of a wide range of microorganisms, including fastidious and non-fastidious bacteria, fungi, and yeasts. Widely used in microbiological testing, sterility testing, and microbial enumeration, TSB is formulated to support robust microbial growth. The medium contains a combination of casein and soya peptones, which provide essential nutrients such as organic nitrogen, amino acids, and peptides. The peptones in this medium offer enhanced growth-promoting properties. Sodium chloride maintains osmotic balance, while glucose and natural sugars from soya peptone serve as fermentable carbohydrate sources. Dipotassium hydrogen phosphate acts as a buffer to stabilize the pH. Tryptic Soy Broth is particularly effective for isolating organisms from blood or other body fluids. It is often used in the tube dilution method for determining antibiotic susceptibility. The addition of a small amount of agar (approximately 0.05–0.2%) renders the broth suitable for cultivating obligate anaerobes, such as Clostridium species. Anticoagulants, such as sodium polyanetholesulfonate or sodium citrate, may be added before sterilization to enhance their utility in blood culture applications. For blood cultures, 5–10 mL of blood can be added to 50 mL of medium. This nutrient-rich medium is an essential tool for a variety of microbiological applications, ensuring reliable and consistent results.

Application

Tryptic Soy Broth is a highly nutrient-rich, general-purpose liquid culture medium designed for the cultivation and enrichment of a wide range of microorganisms, including fastidious and non-fastidious bacteria, fungi, and yeasts. Known for its superior growth performance, TSB is particularly well-suited for supporting the growth of Staphylococcus aureus strains. The medium is widely used in microbiological testing, sterility testing, microbial enumeration, and antibiotic susceptibility testing.

Features and Benefits

Features:

• High-Quality Standards: NutriSelect^® Plus powdered culture media are made from carefully selected raw materials, ensuring consistent quality.
• Wide Range of Applications: Suitable for various microbiological needs, including academic research, food and beverage testing, and pharmaceutical applications.
• Regulatory Compliance: Products meet industry standards and are compliant with ISO 9001 quality assurance.
• Innovative Formulations: Includes novel media types such as non-animal-derived alternatives, synthetically defined media, and chromogenic media.

Benefits:

• Convenience and Safety: Easy-to-use powdered format with minimal clumping and excellent solubility, even in warm or humid conditions.
• Sustainability: Reduced risks associated with fine dust and toxic substance inhalation, ensuring a safer work environment.
• Extended Shelf Life: Products have a prolonged shelf life of up to five years.
• High Reproducibility: Ensures consistent results across batches, exceeding regulatory demands.
• Comprehensive Portfolio: A vast selection of over 350 products tailored to meet diverse microbiological testing needs.

Preparation Note

Suspend 30 g of dehydrated media in 1 litre of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121°C for 15 minutes. Cool to 45-50°C. Mix gently and dispense into sterile Petri dishes or sterile culture tubes.

Other Notes

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)