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Key Documents

TMS-013

Sigma-Aldrich

Fetal Bovine Serum

US Origin, sterile-filtered, suitable for cell culture

Synonym(s):

FBS, FCS, Fetal Calf Sera, Fetal Calf Serum, Fetal bovine Sera, sera, serum

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.75

biological source

bovine fetus

Quality Level

sterility

sterile; sterile-filtered

product line

FBS Classic

form

liquid

composition

hemoglobin, ≤25 mg/dL

manufacturer/tradename

Chemicon®

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

mycoplasma, passes test
virus, tested negative
<10 EU/mL endotoxin

storage temp.

−20°C

General description

Fetal Bovine Serum (FBS) is the ideal cell growth supplement and generally preferred over other types of cell culture sera. Its high levels of nutrients and optimal combination of growth factors makes Fetal Bovine Serum the most effective cell growth promoter for virtually any cell culture system. In addition, the significantly lower content of antibodies compared to adult and newborn sera minimizes the risk of antibody cross reaction with cells in culture.
This serum has been derived from blood aseptically collected in USDA approved abattoirs in the United States of America. This serum was aseptically processed and filter sterilized in the United States. The country of origin of the the crude blood (donor cow origin) is the United States. Each lot is sterile filtered and mycoplasma, endotoxin, and virus tested. A Certificate of Analysis is available upon request.

Application

This serum in standard US Origin FBS and has not been heat inactivated or gamma irradiated.

Quality

See lot specific cofa for complete testing specifications:

Use for general cell culture with common cell lines.

Hemoglobin level: <25.0 mg/dL
Sterility Tested: Pass
Mycoplasma Tested: Pass
Endotoxin level: <10 EU/ml
Negative for BVD, PI3, IBR, Bovine Adeno 3, Bovine Adeno 5, BRSV, REO Virus, Rabies and Blue Tongue
Origin: United States

Storage and Stability

Frozen FBS should be stored at -20ºC until expiration date.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

Related Content

Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.

Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.

Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.

Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.

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