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MAB2083

Sigma-Aldrich

Anti-γ-Catenin Antibody, clone 11E4

clone 11E4, Chemicon®, from mouse

Synonym(s):

Plakoglobin

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

11E4, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... JUP(3728)

Specificity

Produced by immunization with recombinant human G-catenin.

Application

Detect γ-Catenin using this Anti-γ-Catenin Antibody, clone 11E4 validated for use in IP, WB, IC, IH.
Immunohistochemistry: Histochoice fixative is effective.

Immunocytochemistry: Effective for A431 and HT1080 cell lines.

Immunoblotting

Immunoprecipitation

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton

Physical form

Format: Purified
Liquid at 1 mg/mL in 0.02 M phosphate buffer, pH 7.6, 0.25M NaCl, containing 0.1% sodium azide.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to12 months.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Xuming Mao et al.
The Journal of investigative dermatology, 129(4), 908-918 (2008-11-28)
The intercellular interactions of the desmosomal cadherins, desmoglein and desmocollin, are required for epidermal cell adhesion. Pemphigus vulgaris (PV) is a potentially fatal autoimmune blistering disease characterized by autoantibodies against desmoglein (Dsg) 3. During calcium-induced desmosome assembly, treatment of primary
Xuming Mao et al.
The Journal of biological chemistry, 286(2), 1283-1291 (2010-11-17)
Pemphigus vulgaris (PV) is a potentially fatal blistering disease characterized by autoantibodies against the desmosomal adhesion protein desmoglein (Dsg) 3. Whether autoantibody steric hindrance or signaling through pathways such as p38 MAPK is primary in disease pathogenesis is controversial. PV
Sanjay Chauhan et al.
The Journal of biological chemistry, 279(2), 937-944 (2003-10-25)
We recently generated an HT-1080-derived cell line called HT-AR1 that responds to dihydrotestosterone (DHT) treatment by undergoing cell growth arrest in association with cytoskeletal reorganization and induction of neuroendocrine-like cell differentiation. In this report, we show that DHT induces a
Marihan Hegazy et al.
Current protocols in cell biology, 89(1), e115-e115 (2020-10-13)
Biochemical methods can reveal stable protein-protein interactions occurring within cells, but the ability to observe transient events and to visualize the subcellular localization of protein-protein interactions in cells and tissues in situ provides important additional information. The Proximity Ligation Assay®

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