The thickness range of IPFL00010 is 110-145 microns.
IPFL00010
Immobilon® -FL PVDF Membrane
1 roll, 27 cm x 3.75 m, 0.45 µm pore size, transfer membrane with low background fluorescence
Immobilon®-FL PVDF Membrane
Synonym(s):
Western blotting membrane, blotting membrane, transfer membrane
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About This Item
UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric
Pore size:
0.45 μm pore size
Compatibility:
for use with All fluorescence dyes, for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
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Product Name
Immobilon®-FL PVDF Membrane, 1 roll, 27 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.
material
PVDF membrane, plain filter, white filter
feature
hydrophobic
manufacturer/tradename
Immobilon®
technique(s)
dot blot: suitable, western blot: suitable
filter L × W
27 cm × 3.75 m
pore size
0.45 μm pore size
capacity
155 μg/cm2 adsorption capacity (insulin), 205 μg/cm2 adsorption capacity (BSA), 300 μg/cm2 adsorption capacity (goat IgG)
compatibility
for use with All fluorescence dyes, for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
detection method
chemiluminescent, colorimetric, fluorometric
shipped in
ambient
Quality Level
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Related Categories
1 of 4
This Item | |||
|---|---|---|---|
| pore size 0.45 μm pore size | pore size 0.45 μm pore size | pore size 0.45 μm pore size | pore size 0.45 μm pore size |
| material PVDF membrane, white filter, plain filter | material PVDF membrane, plain filter, white filter | material PVDF membrane, plain filter, white filter | material PVDF membrane, plain filter, white filter |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 400 |
| detection method chemiluminescent, fluorometric, colorimetric | detection method chemiluminescent, colorimetric, fluorometric | detection method chemiluminescent, colorimetric, fluorometric | detection method chemiluminescent, colorimetric, fluorometric |
| compatibility for use with All fluorescence dyes, for use with CPTS, for use with Ponceau-S red, for use with Amido black, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Coomassie brilliant blue | compatibility for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red | compatibility for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red | compatibility for use with All fluorescence dyes, for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby |
| manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® |
General description
The Immobilon®-FL transfer membrane is a polyvinylidene fluoride (PVDF) microporous membrane with a nominal pore size of 0.45 μm. It is designed for binding proteins transferred from a variety of gel matrices. The Immobilon®-FL transfer membrane is hydrophobic and offers a uniformly controlled pore structure with a high binding capacity for biomolecules. The membrane offers optimal blotting for proteins with molecular weights greater than 20,000. The Immobilon®-FL membrane displays very low autofluorescence across a wide range of excitation/emission wavelengths. This property makes it ideal for blotting applications involving fluorescence-based immunodetection. Immobilon®-FL membrane can also be used for standard chemiluminescent or chromogenic detection.
Features and Benefits
Immobilon-FL Membrane:
- The first transfer membrane optimized for fluorescence applications; Extremely low background enhances the sensitivity of all fluorescence detection protocols
- Compatible with all commonly used fluorescent probes at a wide range of excitation/emission wavelengths
- Best for multiplexing and chemifluorescence detections
- compatible with standard blocking agents and buffers
Legal Information
Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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S A Misek et al.
Oncogene, 39(7), 1466-1483 (2019-10-30)
Over half of cutaneous melanoma tumors have BRAFV600E/K mutations. Acquired resistance to BRAF inhibitors (BRAFi) remains a major hurdle in attaining durable therapeutic responses. In this study we demonstrate that ~50-60% of melanoma cell lines with vemurafenib resistance acquired in
Guangchuan Wang et al.
Biological research, 52(1), 60-60 (2019-12-19)
Recent studies have confirmed that RASAL1 has an antitumor effect in many cancers, but its functional role and the molecular mechanism underlying in colon cancer has not been investigated. We collected human colon cancer tissues and adjacent non-tumor tissues, human
David J Ferland et al.
PloS one, 15(2), e0229251-e0229251 (2020-02-25)
Since chemerin's identification as an adipokine, it has been associated with a number of human diseases including diabetes and obesity. However, the basic scientific foundation for these clinical determinations is still lacking. Fibroblastic mouse 3T3 cells are unable to develop
Enrique Calvo et al.
Science advances, 6(26), eaba7509-eaba7509 (2020-07-09)
Mitochondrial respiratory complexes assemble into supercomplexes (SC). Q-respirasome (III2 + IV) requires the supercomplex assembly factor (SCAF1) protein. The role of this factor in the N-respirasome (I + III2 + IV) and the physiological role of SCs are controversial. Here
Daniel Del Valle Morales et al.
Nucleic acids research, 48(7), 3806-3815 (2020-01-31)
Cap homeostasis is the cyclical process of decapping and recapping that maintains the translation and stability of a subset of the transcriptome. Previous work showed levels of some recapping targets decline following transient expression of an inactive form of RNMT
Related Content
Antibody reuse for Western blotting is a common practice for many researchers. While many antibodies lose potency with time or degrade even faster due to improper storage conditions, it is important to recognize the potential value of recovering the primary antibody for possible reuse in some experiments. The SNAP i.d.® 2.0 system is not only able to reduce the immunodetection processing time, but its flexibility lets you combine conditions used in the standard immunodetection protocol and also allows the collection of antibody for future reuse. Here, we compare the antibody recovery and reuse in the standard immunodetection protocol with the antibody recovery and reuse in SNAP i.d.® system using the extended protocol and the original SNAP i.d.® protocol.
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What is the thickness of this product?
1 answer-
Helpful?
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Is it possible to use Immobilon FL instead of Immobilon P for western blots?
1 answer-
Yes, Immobilon FL can be used as an alternative to Immobilon P for western blots.
Helpful?
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Is 0.22 µm PVDF membrane available for fluorescent detection in Western blotting?
1 answer-
Immobilon PSQ, with a pore size of 0.2 µm, is suitable for various detection methods including fluorescent, radioactive, chromogenic, and chemifluorescent detection. But when using Immobilon PSQ in Western Blotting, it is important to consider that there may be increased background noise, which might require more stringent wash steps to ensure accurate results.
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Active Filters
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Great for western blots
I have been using this product for close to two and half years now. It is a highly reliable PVDF roll for western blotting. I use it to transfer a wide variety of proteins and use HRP conjugated secondary antibodies for imaging bands. The results have always been sharp, reliable and most importantly, reproducible. I shall buy again when we run out.
- A donation to charity was made for this review
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