Skip to Content
Merck
All Photos(2)

Documents

ABS229

Sigma-Aldrich

Anti-HMG-CoA reductase Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

3-hydroxy-3-methylglutaryl-coenzyme A reductase, HMG-CoA reductase

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

primate (based on 100% sequence homology)

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... HMGCR(3156)

General description

The 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase) enzyme is a ubiquitously expressed glycoprotein that is bound to the membrane of the endoplasmic reticulum (ER), but also projects an active C-terminal catalytic tail into the cytoplasm. It is the rate-limiting enzyme in the mevalonate pathway as it catalyzes the conversion of HMG CoA to mevalonate, which is a critical precursor protein in the synthesis of sterols such as cholesterols. In mammalian cells, HMG-CoA reductase is regulated by multiple mechanisms. It is downregulated by high levels of exogenous cholesterol bound to the low density lipoprotein. It is also regulated by sterol and nonsterol metabolites of mevalonate which may exert inhibitory effects at the transcriptional level. Previous studies have suggested that sterols may inhibit the sterol regulatory element-binding proteins (SREBPs) which function as transcription factors that enhance the expression of genes required for sterol biosynthesis. The end-stage degradation process may be mediated by the ubiquitin degradation system. The inhibition of HMG-CoA reductase has been widely studied for the treatment of cholesterol-related conditions.

Specificity

Other homologies: Porcine (85% sequence homology).
This antibody recognizes HMG CoA reductase at the linker domain.

Immunogen

Epitope: Linker domain
KLH-conjugated linear peptide corresponding to the linker domain of human HMG CoA reductase.

Application

Anti-HMG-CoA reductase Antibody detects level of HMG-CoA reductase & has been published & validated for use in Immunoprecipitation, Western Blotting.
Immunoprecipitation Analysis: 10 µg/mL from a representative lot immunoprecipitated HMG CoA reductase from HepG2 cell lysate.

Quality

Evaluated by Western Blot in HepG2 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected HMG CoA reductase on 10 µg of HepG2 cell lysate.

Target description

~97 kDa observed

Linkage

Replaces: 07-572

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Yifan Kong et al.
The Journal of biological chemistry, 293(37), 14328-14341 (2018-08-10)
Enzalutamide, a nonsteroidal second-generation antiandrogen, has been recently approved for the management of castration-resistant prostate cancer (CRPC). Although patients can benefit from enzalutamide at the beginning of this therapy, acquired enzalutamide resistance usually occurs within a short period. This motivated
Ning Liang et al.
Molecules (Basel, Switzerland), 26(12) (2021-07-03)
Rutin (R) and quercetin (Q) are two widespread dietary flavonoids. Previous studies regarding the plasma cholesterol-lowering activity of R and Q generated inconsistent results. The present study was therefore carried out to investigate the effects of R and Q on
Kim Loh et al.
Hepatology communications, 3(1), 84-98 (2019-01-09)
Adenosine monophosphate-activated protein kinase (AMPK) regulates multiple signaling pathways involved in glucose and lipid metabolism in response to changes in hormonal and nutrient status. Cell culture studies have shown that AMPK phosphorylation and inhibition of the rate-limiting enzyme in the
Slawomir Mandziuk et al.
Basic & clinical pharmacology & toxicology, 119(3), 330-340 (2016-03-19)
Tirapazamine is a hypoxia-activated prodrug which was shown to exhibit up to 300 times greater cytotoxicity under anoxic in comparison with aerobic conditions. Thus, the combined anticancer therapy of tirapazamine with a routinely used anticancer drug seems to be a
Beth Buchanan et al.
PloS one, 13(3), e0194979-e0194979 (2018-03-27)
The natural alkaloid berberine has been ascribed numerous health benefits including lipid and cholesterol reduction and improved insulin sensitivity in diabetics. However, oral (PO) administration of berberine is hindered by poor bioavailability and increasing dose often elicits gastro-intestinal side effects.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service